| 人外周血树突状细胞-乳腺癌细胞融合细胞 |
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| 引用本文： | 伊焕发,唐军民,唐岩,孙红亚,甄昱,戴燕,秦屹. 人外周血树突状细胞-乳腺癌细胞融合细胞[J]. 解剖学杂志, 2003, 26(2): 105-108 |
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| 作者姓名： | 伊焕发 唐军民 唐岩 孙红亚 甄昱 戴燕 秦屹 |
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| 作者单位： | 1. 北京大学基础医学院组织胚胎学教研室,北京,100083
2. 中国人民解放军261医院妇产科 |
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| 基金项目： | 国家自然科学基金(39970380) |
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| 摘 要： | 目的：探讨树突状细胞—肿瘤细胞融合细胞的形态特性，为研制融合细胞疫苗提供形态学依据。方法：将免疫磁珠法分离的人外周血树突状细胞与人乳腺癌细胞株MCF7融合，瑞氏—姬姆萨染色观察；扫描电镜观察树突状细胞、融合细胞的表面超微结构。结果：树突状细胞与MCF细胞按10：1比例融合后，一个乳腺癌细胞可以与一个或多个树突状细胞相融合；扫描电镜下可见分离的树突状细胞表面有突起，树突状细胞/MCF7融合细胞具两种亲代细胞的表面超微结构特点。结论：树突状细胞与人乳腺癌细胞融合后无明显的形态改变。
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| 关 键 词： | 乳腺癌细胞 树突状细胞 融合细胞 疫苗 免疫磁珠法 瑞氏－姬姆萨染色法 扫描电镜 |
Scanning electron microscopy of fusion cells by dendritic cells in peripheral blood with human breast carcinoma cells |
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| YI Huan-Fa,TANG Jun-Min,TANG Yan,SUN Hong-Ya,ZHEN Yu,DAI Yan,QIN Yi. Scanning electron microscopy of fusion cells by dendritic cells in peripheral blood with human breast carcinoma cells[J]. Chinese Journal of Anatomy, 2003, 26(2): 105-108 |
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| Authors: | YI Huan-Fa TANG Jun-Min TANG Yan SUN Hong-Ya ZHEN Yu DAI Yan QIN Yi |
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| Abstract: | Objective:To study morphological features of the fusion cells and provide a morphological basis for producing fusion cell vaccine. Methods; Dendritic cells acquired by MACS from human peripheral blood were fused with human breast carcinoma cell line,MCF7. The morphological characteristics of dendritic cells, MCF7 cells and dendritic cell/MCF7 cell were observed with Wright-Giemsa staining and scanning electron microscopy(SEM). Results: A MCF7 cell could fuse with one or more dendritic cells. Process of dendritic cells could be observed by SEM. The fusion cells retained its parental cells' surface ultrastructural feature. Conclusion:The morphological characteristics of the fusion cells of dendritic cells with MCF7 cells do not alter compared with its parental cells. |
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| Keywords: | dendritic cell breast carcinoma cell fusion cell |
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