WT1基因对肾胚瘤细胞系G401细胞肿瘤逆转的研究

目的 肾胚瘤是一种较常见的儿科实体肿瘤，该肿瘤的发生与抑癌基因WT1密切相关。本研究的目的是利用脂质体介导转染法将WT1cDNA导入G401细胞，研究WT1基因对G401细胞生长凋亡及致瘤性的作用。方法 通过Western blot检测WT1基因在肾胚瘤中的表达、采用碘化丙锭(PI)染色法检测G401细胞的凋亡情况、并且对G401的致瘤性进行分析，探讨WT1基因在肾胚瘤发生及治疗中的作用。结果 WT1 DNA可诱导G401细胞凋亡，并且使其致瘤性有所降低。结论 该结果为确定WT1在肾胚瘤病因方面的作用、为肾胚瘤的基因治疗提供重要的依据。

WT1 expression alters tumorigenicity of the G401 cell line

Objective Wilms tumor is one of the most common solid tumors in children.Some studies have implicated a loss of WT1 tumor suppressor gene function in the development of Wilms'tumor.The objective was to determine the potential biological consequences of WT1 in these tumors.Methods We transfected WT1 cDNA into the pediatric kidney-derived cell line G401.Ddetecting WT1 gene expression by Western blot,tumorigenicity and apoptosis of G401 cell line. Results Introduction of this gene caused a significant suppression of tumorigenicity in the cells and apoptosis cell number increased.Conclusion These data indicafe the concept for a central role for WT1 inactivation in the etiology of this disease.