苦丁茶对豚鼠离体气管平滑肌收缩功能的影响

目的 :研究苦丁茶对豚鼠离体气管平滑肌的影响。方法 :以累积技术建立CaCl₂ 和组织胺 (His)的量效曲线 ,研究了苦丁茶对CaCl₂ ,His和乙酰胆碱 (Ach)等致喘介质对豚鼠离体气管平滑肌收缩功能的影响。结果 :CaCl₂ 和His引起气管条明显的收缩 ,pD₂ 分别为 3.59和 5 .34。苦丁茶能明显压低CaCl₂ 和His的量效曲线 ,并使曲线右移。苦丁茶对Ach和His 3×10^-6mmol·L^-1引起的气管平滑肌收缩有明显的逆转作用 ,IC₅₀ 分别为 0.16mg·ml^-1和 0.2 1mg·ml^-1。结论 :提示苦丁茶有明显的舒张豚鼠离体气管平滑肌的作用。

The Effect of Kudingcha (Ilex latifolia Thunb.) on the Contraction of Isolated Guinea-pig Tracheal Smooth Muscle in Vitro

Objective: To study the effects of Kudingcha ( Ilex latifolia Thunb., IL) on the isolated guinea pig tracheal smooth muscle in vitro. Method: By obtaining CaCl₂ and Histamine accumulative dose response curves, to observe the influences of IL on the contraction of tracheal strips induced by calcium and some asthmogenic mediators. Result: CaCl₂ and histamine caused significant contraction of tracheal smooth muscle and pD 2 was 3.55 and 5.34 respectively. After incubated with IL, the dose response curves of CaCl₂ and histamine were significantly shifted to the right, and the maximal contractile force was reduced. IL could also inhibit isolated tracheal strip contraction induced by acetylcholine 3×10 -6 mol·L^-1 and histamine 3×10^-6 mol·L ^-1 , and IC₅₀ was 0.16mg·ml^-1 and 0.21mg·ml^-1 . Conclusion: Kudingcha has significant dilated effects on tracheal smooth muscle.