浙江省丽水市首起非O1群霍乱弧菌食物中毒分子生物学溯源分析

目的 利用分子生物技术了解引起食物中毒的非O1群霍乱弧菌携带毒力基因情况及耐药性特征.方法 对检出的非O1群霍乱弧菌菌株进行耐药性分析,同时采用聚合酶链反应(PCR)方法进行霍乱弧菌肠毒素基因(Ctx)、小带联结毒素基因(Zot)、辅助霍乱肠毒素(Ace)基因的检测,并参照美国CDC PulseNet的统一方法进行脉冲场凝胶电泳(PFGE)分型并作同源性分析.结果 从8例患者和8份食品标本中分离到7株非O1群霍乱弧菌,未检出Ctx、Zot、Ace等毒素基因,检出溶血素,Dienes试验和脉冲场凝胶电泳图谱显示病人分离株和剩余食物检出株同源.经耐药性分析,菌株除对复方新诺明、氨苄西林100%耐药外,对其余抗生素均较敏感.结论 通过实验证实本次食物中毒是由聚餐者共同食用冷菜凤爪所致,流行病学追溯从患者共同食用冷菜和海产品中非O1群霍乱弧菌毒力基因、耐药性和脉冲场凝胶电泳分析为同源菌株.

Molecular biological source analysis of the initial Vibrio cholera non-O1 food poisoning in Lishui City

Objective This study was conducted to determine the characteristics of antibiotics resistance and virulence gene of Vibrio cholera non-O1 which caused food poisoning through molecular biological methods. Method Antibiotics resistance analysis on Vibrio cholera non-O1 isolates was performed with drug sensitivity test. The virulence gene Ctx, Zot and Ace were detected by PCR. The molecular types were then classified by pulsed field gel electrophoresis (PFGE) and homology analysis was conducted. Results A total of 7 Vibrio cholera non-O1 strains were isolated from 8 patients and 8 food samples. Virulence gene analysis showed that Vibrio cholera non-O1 isolates carried none of 3 virulence genes(Ctx、Zot、Ace). All the isolates were positive to hemolysin. Classification of molecular types with PFGE and Dienes Test showed the isolates from patients and those from leaving food samples were homologous. The antibiotics resistance analysis showed that the isolates were sensitive to almost all the tested antibiotics except ampicillin and SMZco(resistance rates were 100%). Conclusions This experiment confirmed that the food poisoning occurred from eating were confused chicken claw. Retrospective epidemiology revealed homologous strains from the virulence genes of Vibrio Cholerae non-O1 in remaining cold dish and seafood, resistance and PFGE analysis.