神经生长因子对小鼠海马突触体内[Ca2+]i的影响

目的探讨神经生长因子(NGF)对小鼠海马突触体内[Ca2+]i水平的影响。方法采用海马内微注射方法，使用荧光指示剂Fura-2／AM检测各组小鼠海马突触体内[Ca2+]i水平。结果1．NGF对青年小鼠海马突触体内[Ca2+]i水平没有显著影响；2．NGF能缓解衰老小鼠海马突触体内[Ca2+]i超载现象；3．NGF也能降底人为海马突触体内[Ca2+]；水平。结论NGF通过调节海马突触体内[Ca2+]i的水平来改善衰老动物的学习记忆能力。

Effect of Nerve Growth Factor on Intrasynaptosomai Free Calcium Ion Concentration of Hippocampus in Mice

Objective To study the effect of nerve growth factor on the intrasynaptosomal free calcitun ion concentration of the hippocampus in mice. Methods The microinjection of NGF into the bilateral intrahippocampi of mice was applied. The intrasynaptosomal free calcium ion concentration of the hippocampus was determined with fluorescence indicator Fura-2/AM by using the spex cation measurement system. Results 1.There was no detectable effect of NGF on intrasynaptosomal free calcium ion concentration of the hippocampus in young mice (1-month-old). 2. The over- concentration of calcium ions in the hippocampi in aged mice (18-month-old) could be decreased significantly. 3. The artificial high level of calcium ions in the hippocampi of young mice could also be reduced obviously. Conclusion Microinjection of NGF via the hippocapus could alleviate Ca2 - overload in the hippocampus of aged mice and reduce the artificially caused high Ca2 -level in the hippocampus of young mice.