新型冠状病毒肺炎患者IgM抗体与血细胞计数及C反应蛋白的相关性研究

目的 探讨白细胞计数、C反应蛋白及新型冠状病毒(SARS-CoV-2)特异性IgM抗体在新型冠状病毒感染的肺炎(COVID-19)患者临床诊断中的应用价值。方法 对59例COVID-19确诊患者疾病早期的外周血白细胞、淋巴细胞及C反应蛋白进行检测分析,并采用胶体金免疫层析技术检测不同病程患者血清中SARS-CoV-2特异性IgM抗体的水平。结果①59例确诊患者中,白细胞计数正常或降低者为57例(96.61%),淋巴细胞计数降低为20例(33.9%),C反应蛋白升高者为41例(69.49%)。COVID-19患者与健康人群相比,外周血白细胞、淋巴细胞及C反应蛋白的差异有统计学意义(P< 0.05);②59例确诊患者中,52例(88.14%)患者血清SARS-CoV-2特异性IgM抗体检测为阳性,健康体检者IgM抗体均为阴性。COVID-19患者在病程第(0~6)d、(7~10)d、(11~15)d、(16~20)d血清SARS-CoV-2特异性IgM抗体阳性率分别为25%、81.82%、90%、100%。③IgM阳性组与IgM阴性组比较,外周血白细胞、淋巴细胞及C反应蛋白水平的差异均无统计学意义(P>0.05)。结论 ①机体感染SARS-CoV-2后,外周血白细胞计数一般正常或减低,部分患者出现淋巴细胞减少,常伴有C反应蛋白升高。动态观察白细胞、淋巴细胞和C反应蛋白的变化,对于COVID-19的临床诊断和病情观察具有重要意义。②胶体金免疫层析技术检测患者血清中的SARS-CoV-2特异性IgM抗体可用于SARS-CoV-2感染患者的辅助诊断。

Study on the Correlation between IgM Antibody and Bloodcell Count and C-reactive Protein in Patientswith COVID-19 Convalescent Plasma

Objective To investigate the clinical value of blood cell count,C-reactive protein and novel coronavirus(SARS-CoV-2)specific IgM antibody in the diagnosis of COVID-19.Methods The peripheral blood leukocyte,lymphocyte counts and C-reactive protein in 59 patients diagnosed with COVID-19 were detected and analyzed,and the level of SARS-CoV-2 specific IgM antibody in serum of patients with different course of disease were detected by colloidal gold immunochromatography.Results①Among the 59 patients,57(96.61%)had normal or decreased leukocyte count,20(33.9%)had decreased lymphocyte count and 41(69.49%)had increased C-reactive protein.Compared with the healthy people,there were significant differences in the counts of leukocytes,lymphocytes and C-reactive protein in the patients with COVID-19(P<0.05).②Ofthe 59 confirmed patients,52(88.14%)had positive SARS-CoV-2 specific IgM antibody in their serum,while the healthy subjects had negative IgM antibody.In patients with COVID-19,the positive rates of serum SARS-CoV-2 specific IgM antibodies were 25%,81.82%,90%,and 100%during the course of 0-6 d,7-10 d,11-15 d,16-20 d,respectively.③There was no significant difference in the levels of peripheral blood leukocytes,lymphocytes and C-reactive protein between the IgM positive group and the IgM negative group(P>0.05).Conclusion①After infection with SARS-CoV-2,the leukocyte count in peripheral blood was generally normal or decreased,and some patients presented lymphocytopenia,often accompanied by elevated C-reactive protein.Dynamic observation of the changes of leukocytes,lymphocytes and C-reactive protein is of great significance for the clinical diagnosis and disease observation of COVID-19.②The detection of SARS-CoV-2 specific IgM antibodies in the serum of patients by colloidal gold immunochromatography can be used for auxiliary diagnosis of patients with SARS-CoV-2 infection.