非小细胞肺癌中GPR120、VEGF的表达及临床意义

目的探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)中GPR120、VEGF的表达及与预后的关系。方法应用qPCR及Western blot法检测42例NSCLC新鲜组织(包括癌组织和对应癌旁组织)中GPR120、VEGF mRNA及蛋白表达。免疫组化检测85例NSCLC癌组织及62例癌旁组织石蜡标本中GPR120、VEGF蛋白的表达,并分析其表达与患者生存期的关系。结果NSCLC组织中GPR120、VEGF的表达明显高于对应癌旁组织(P<0.05)。GPR120表达与淋巴结转移、远处转移、TNM分期密切相关(P<0.05)。VEGF表达与淋巴结转移、TNM分期密切相关(P<0.05)。qPCR及免疫组化结果均提示GPR120与VEGF表达显著相关(r=0.7386,P<0.0001;r=0.413,P<0.001)。NSCLC患者生存期与GPR120低表达、VEGF低表达呈正相关(P<0.05)。GPR120蛋白高表达、淋巴结转移、远处转移均为NSCLC患者预后的危险因素(P<0.05)。结论GPR120、VEGF在NSCLC的侵袭、转移中发挥重要作用,并且GPR120可作为NSCLC有价值的预后生物学标志物。

Expression and clinical significance of GPR120 and VEGF in non-small cell lung cancer

Purpose To investigate the expression of G-protein coupled receptor 120(GPR120)and vascular endothelial growth factor(VEGF)in non-small cell lung cancer(NSCLC)tissues and their relationship with prognosis.Method The expressions of GPR120,VEGF mRNA and protein in 42 cases of NSCLC fresh lung cancer tissues(including cancer tissues and matched paracancerous tissues)were analyzed by qPCR and Western blotting.Immunohistochemistry was used to detect the expression of GPR120 and VEGF protein in paraffin specimens of 85 cancer tissues and 62 paracancerous tissues of NSCLC,and the relationship between their expression and patient survival was analyzed.Result The expression of GPR120 and VEGF in NSCLC tissues was significantly higher than matched paracancerous tissues,and the difference was statistically significant(P<0.05).GPR120 expression was closely related to lymph node metastasis,distant metastasis,and TNM stage(P<0.05).The expression of VEGF was closely related to lymph node metastasis and TNM stage(P<0.05).Both qPCR and immunohistochemical results indicated that GPR120 was significantly correlated with VEGF expression(r=0.7386,P<0.0001,r=0.413,P<0.001).The survival of NSCLC patients was positively correlated with low GPR120 expression and low VEGF expression(P<0.05).High GPR120 protein expression,lymph node metastasis,and distant metastasis were all risk factors for prognosis in patients with NSCLC(P<0.05).Conclusion GPR120 and VEGF may play an important role in the invasion and metastasis of NSCLC,and GPR120 can be a valuable prognostic biomarker for patients with NSCLC.