灵菌红素通过促进Bim调控人乳腺癌细胞凋亡

目的探讨Bim在灵菌红素调控人乳腺癌MCF-7细胞增殖和凋亡中发挥的作用。方法培养人乳腺癌MCF-7细胞、采用灵菌红素处理MCF-7细胞,应用MTT检测细胞活力增殖及流式细胞计数检测细胞凋亡率,RT-PCR和Western blot检测Bim mRNA和蛋白表达情况。结果灵菌红素能抑制MCF-7细胞增殖,并诱导MCF-7细胞凋亡,效果呈时间和浓度依赖性(P0.05)。灵菌红素处理后MCF-7细胞S期显著减少、增殖指数下降,G1期显著增加(P0.05)。灵菌红素促进MCF-7细胞内促凋亡蛋白Bim表达升高,细胞凋亡率亦显著上升;沉默Bim能拮抗灵菌红素对MCF-7细胞凋亡的促进作用(P0.05)。结论灵菌红素通过上调Bim从而抑制人乳腺癌MCF-7细胞增殖和促进其凋亡。

Role of Bim on the proliferation and apoptosis of breast cancer cells induced by prodigiosin

［Abstract］ Objective To investigate the effects and mechanism of prodigiosin in regulation of breast cancer cells. Methods MTT and flow cytometry were used to detect the proliferation and apoptosis of human breast cancer MCF-7 cells. Bim was detected by RT-PCR and Western blot. siRNA was used to investigate the role and mechanism of prodigiosin on the apoptosis of MCF-7 cells induced by prodigiosin. Results Prodigiosin inhibited the proliferation of MCF-7 cells and induced the apoptosis of MCF-7 cells in a dose and time dependent manner. Prodigiosin arrested MCF-7 cells at G1 phase， with reduced proliferation index and decreased cells in S phase. Prodigiosin induced the expression of Bim in MCF-7 cells in a concentration and time dependent manner. Suppression of Bim can partially abolish the apoptosis induction of MCF-7 cells by prodigiosin. Conclusion Prodigiosin can inhibit the proliferation of MCF-7 cells， and induce MCF-7 cells apoptosis through the regulation of Bim.