T cell subpopulation phenotypes in filarial infections: CD27 negativity defines a population greatly enriched for Th2 cells |
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Authors: | Elson Lynne H; Shaw Stephen; Van Lier Rene A W; Nutman Thomas B |
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Institution: | Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases Bethesda, MD 20892, USA
1 Laboratory of Experimental Immunology, National Cancer Institute Bethesda, MD 20892, USA
2 Central Laboratory of the Netherlands Red Cross Blood Transfusion Service Amsterdam, The Netherlands |
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Abstract: | Three-color flow cytometric analysis was used to define surfacemarkers which identify the Th2-type CD4+ cells responsible forthe eosinophilia and elevated serum IgE typical of tissue invasivehelminth infections. A group of six mAba to well known cellsurface markers were screened for differential expression onCD4+ CD45RO+ lymphocytes from normal individuals (NL; n = 6)and filaria-infected patients (PT; n = 10). The majority ofmarkers were expressed equally by both groups, but the CD4+CD45RO+ cells in the PTs showed significantly higher levelsof expression of HLA-DR than those of NLs (P = 0.014). ThisCD4+ HLA-DR+ subpopulation was then studied further for itsexpression of an additional 10 activation and adhesion molecules.CD27 showed a trend for lower intensities of expression on PTCD4+ HLA-DR+ cells than on those of NLs. Analysis of the serumfrom both NLs and PTs revealed that PTs had significantly higherlevels of soluble CD27 and CD25 (IL-2R) In the serum than NLs(P < 0.01 and P = 0.022 respectively) indicating a generalstate of Immune activation and differentiation. Functional analysisof the CD4+ HLA-DR+ and the CD4+ CD27– subpopulatlonsrevealed that the CD4+ HLA-DR+ cells produced significantlyhigher levels of IL-5 than the CD4+ HLA-DR– cells (P <0.04), and the CD4+ CD27– cells produced significantlyhigher levels of both IL-4 and IL-5 than the CD4+ CD27–cells (P <0.05 and P <0.001 respectively). Thus, whilethe CD4+ CD27– and CD27+ subpopulatlons contain Th1 andTh0 cells, only the CD4+ CD27– population contains theTh2 cells (producing both IL-4 and IL-5). |
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Keywords: | CD27 filariasis IL-4 IL-5 lymphocyte markers |
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