Chrom ID ESBL显色平板对产ESBLs肠杆菌科细菌筛选作用的评估 |
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引用本文: | 韩立中,杨海慧,祝艳萍,蒋晓飞,张秀珍,倪语星. Chrom ID ESBL显色平板对产ESBLs肠杆菌科细菌筛选作用的评估[J]. 上海医学检验杂志, 2010, 0(2) |
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作者姓名: | 韩立中 杨海慧 祝艳萍 蒋晓飞 张秀珍 倪语星 |
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作者单位: | 上海交通大学医学院附属瑞金医院;上海交通大学医学院附属仁济医院;复旦大学附属华山医院;卫生部北京医院; |
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基金项目: | 上海市自然科学基金项目(09ZR1419200); 卫生部卫生公益性行业科研专项经费项目(200802107) |
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摘 要: | 目的评估新的产色培养基Chrom ID ESBL在检测产超广谱β-内酰胺酶(ESBLs)肠杆菌科细菌中的可靠性。方法用2组肠杆菌科细菌进行评估,一组为285株上海地区检出的临床分离株,这些菌株已用美国临床实验室标准化研究所(CLSI)推荐的确证试验确认了是否产ESBLs,另一组28株产ESBLs肠杆菌科细菌,在以前的研究工作中已确认了这些菌株的产酶特性和ESBLs基因型别。结果Chrom ID ESBL能有效地检测产ESBLs的肠杆菌科细菌。结论Chrom ID ESBL是一种可靠的产ESBLs肠杆菌科细菌的筛选培养基。
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关 键 词: | Chrom ID ESBL显色平板 超广谱β-内酰胺酶 肠杆菌科 评估 |
Evaluation of ChromID ESBL medium for screening of ESBLs-producing Enterobacteriaceae |
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Abstract: | Objective To evaluate the reliability of a new selective chromogenic agar medium,ChromID ESBL,for detection of extended-spectrum beta-lactamases(ESBLs)-producing Enterobacteriaceae.Methods Two arrays of isolates were used to evaluate the reliability of ChromID ESBL.One array contained 285 clinical isolates in which the ESBLs-producing feature was screened by confirmatory tests recommended by Clinical and Laboratory Standards Institute(CLSI).The other array contained 28 enterobacterial isolates with their ES... |
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Keywords: | ChromID ESBL medium Extended-spectrum beta-lactamases Enterobacteriaceae Evaluation |
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