提高精脒/精胺N1乙酰基转移酶表达水平对前列腺癌细胞活性的影响

目的 研究提高前列腺癌细胞（PC3）中精脒/精胺N¹乙酰基转移酶（spermidine/spermine N¹ acetyhransferase，SSAT）表达水平对细胞增殖及侵袭和转移的影响。方法 腺病毒载体Ad-SSAT感染PC3细胞，Western blot法检测细胞中SSAT的表达水平，CCK-8法和克隆形成法检测细胞的增殖能力和克隆形成能力，划痕修复实验和Transwell跨膜实验检测细胞的转移能力。结果 Ad-SSAT组细胞的SSAT水平显著高于对照组（P<0.05）；培养48h后，Ad-SSAT组细胞的增殖能力较对照组和Ad-GFP组有所降低，差异有统计学意义（P<0.05）。克隆形成实验显示，Ad-SSAT组的克隆形成率（14.3%）显著低于对照组（39.7%）和Ad-GFP组的（40.2%）。划痕修复实验显示对照组和Ad-GFP组的迁移率没有明显差异，而Ad-SSAP组迁移速度较慢，在两个时间点均显著低于对照组，差异具有统计学意义（P<0.05）。Transwell跨膜实验，可见对照组与Ad-GFP组跨膜细胞数差异无统计学意义（P<0.05）；Ad-SSAP组跨膜细胞明显低于对照组，差异有统计学意义（P<0.05）。结论 提高前列腺癌细胞中SSAT表达能抑制细胞生长，降低细胞的迁移能力。

Influence of Elevated SSAT Level on the Activity of Prostate Cancer Cell PC3

Objective To explore the influence of elevated spermidine/spermine N¹ acetyhransferase(SSAT) level on the activity of prostate cancer cell PC3. Methods PC3 was infected with adenovirus vector Ad-SSAT, and the level of SSAT was determined by western blot. CCK-8 and clone formation assay were used to test the proliferation and clone formation rate. Wound healing assay and transwell migration assay were applied to analyze the metastasis ability of the cell. Results The level of SSAT was elevated obviously compared with control group(P<0.05). After cultured for 48h, the proliferation rate of cell in Ad-SSAT group was declined compared with the Ad-SSAT group and control group(P<0.05). The clone formation assay showed that the clone formation rate(14.3%) was much lower than in the Ad-GFP and control group(39.7% and 40.2% respectively). The wound healing assay revealed that there was no difference between the wound healing rate in the control group and Ad-GFP group(P>0.05), and it was much lower in the Ad-SSAT group after 6 and 12h, the difference was statistically significant(P<0.05). The transwell migration assay revealed that the metastasis ability was statistically declined compared with the Ad-GFP and the control group(P<0.05). Conclusion Elevated the expression level of SSAT in PC3 can inhibit the proliferation, metastasis ability.