黄芪注射液对乳腺癌MCF-7细胞增殖周期以及细胞凋亡、迁移影响的实验研究

目的 探讨黄芪注射液对乳腺癌MCF-7细胞增殖、周期、凋亡及迁移的影响。方法 通过MTT法观察药物处理后对乳腺癌MCF-7细胞的生长增殖情况的影响；通过流式细胞术检测黄芪注射液对乳腺癌MCF-7细胞增殖周期、凋亡的影响；利用划痕实验检测黄芪注射液对乳腺癌MCF-7细胞的迁移的影响。结果 MTT结果发现，不同浓度的黄芪注射液（100、200、400、600、800mg/ml）对乳腺癌MCF-7细胞的增殖具有不同程度的抑制作用；流式细胞术结果显示，与空白对照组相比，200和400mg/ml黄芪注射液均能导致G₁期增加，且两个浓度的凋亡率都大于空白对照组；划痕实验结果显示200和400mg/ml黄芪注射液组较空白对照组划痕愈合率低。结论 不同浓度的黄芪注射液在体外对乳腺癌MCF-7细胞增殖具有一定的抑制作用；同时可将细胞增殖周期阻滞在G₁期，促进MCF-7细胞凋亡；一定浓度的黄芪注射液对MCF-7细胞的迁移具有抑制作用，为乳腺癌的临床应用提供实验基础。

Effects of Astragalus Injection on Breast Cancer Cells Proliferation and Apoptosis and Migration

Objective To investigate the effect of Astragalus injection on cell proliferation, cell cycle, apoptosis and migration of breast cancer MCF-7 cells in vitro. Methods MTT method was used to observe cell proliferation of MCF-7 treated with Astragalus injection; Flow cytometry was applied to detect cell cycle and apoptosis of MCF-7 cells; Scratches test was used to study cell migration in MCF-7 cells. Results MTT results showed that cell proliferation in MCF-7 cells treating with different concentrations of Astragalus injection(100, 200, 400, 200, 800mg/ml) could be inhibited. Flow cytometry results indicated that the percentage of G₁ phase in MCF-7 cells treated by 200mg/ml and 400mg/ml Astragalus injection, compared with the control group, could be increased; Moreover, we could observed that apoptosis rate was greater than the control group. The scratch analysis indicated that MCF-7 cells treated by 200mg/ml and 400mg/ml Astragalus injection had lower wound healing rate than control. Conclusion We found Astragalus injection could inhibit cell proliferation and cell migration in different concentration in vitro. Interesting, G₁ phase arrest could be induced and apoptosis rate in MCF-7 cells could be increased by Astragalus injection. Our findings can provide experiment foundation about application of Astragalus injection in clinic medicine.