| 脂氧素A4对缺氧状态下人肝癌细胞增殖的影响 |
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| 引用本文： | 苗烁,王素梅,程雪,李尧丰,马明洋,李钢,吴萍. 脂氧素A4对缺氧状态下人肝癌细胞增殖的影响[J]. 转化医学杂志, 2017, 6(1): 16-19 |
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| 作者姓名： | 苗烁 王素梅 程雪 李尧丰 马明洋 李钢 吴萍 |
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| 作者单位： | 华中科技大学同济医学院基础医学院病理生理学系,华中科技大学同济医学院基础医学院病理生理学系,华中科技大学同济医学院基础医学院病理生理学系,华中科技大学同济医学院基础医学院病理生理学系,华中科技大学附属同济医院肝脏外科中心,华中科技大学同济医学院附属梨园医院外科,华中科技大学同济医学院基础医学院病理生理学系 |
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| 基金项目： | 国家自然科学基金面上项目(81272313) |
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| 摘 要： | 目的探讨缺氧环境下脂氧素A4(lipoxin A4,LXA4)对肝癌细胞增殖的影响。方法采用小鼠肝癌细胞H22接种构建小鼠肝癌皮下移植瘤模型，观察LXA4对肿瘤生长的影响。采用免疫组织化学法检测细胞增殖核抗原Ki67和缺氧诱导因子1α(hypoxia inducible factor1α,HIF1α)表达。四甲基偶氮唑盐比色法检测细胞增殖水平。结果在接种肝癌细胞H22的7～15 d，小鼠皮下可见清晰肿瘤包块生长；在各相应时间点，给予LXA4的小鼠肿瘤体积均明显缩小(P<0.05)，同时明显降低了肿瘤组织内部Ki-67和HIF-1α表达。体外培养发现200 nmol/L LXA4可明显抑制缺氧条件下人肝癌细胞HepG2的增殖。结论LXA4能抑制缺氧条件下肝癌细胞的增殖。
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| 关 键 词： | 脂氧素A4；肝细胞癌；缺氧；增殖 |
The effect of lipoxin A4 on the proliferation of human hepatocellular carcinoma under hypoxia |
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| MIAO Shuo,WANG Sumei,CHENG Xue,LI Yaofeng,MA Mingyang,LI Gang and WU Ping. The effect of lipoxin A4 on the proliferation of human hepatocellular carcinoma under hypoxia[J]. Translational Medicine Journal, 2017, 6(1): 16-19 |
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| Authors: | MIAO Shuo WANG Sumei CHENG Xue LI Yaofeng MA Mingyang LI Gang WU Ping |
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| Affiliation: | Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology,Wuhan Hubei 430030, China,Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology,Wuhan Hubei 430030, China,Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology,Wuhan Hubei 430030, China,Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology,Wuhan Hubei 430030, China,Hepatic Surgery Center, Tongji Hospital of Tongji Medical College,Huazhong University of Science and Technology, Wuhan Hubei 430030, China;,Department of Surgery,Liyuan Hospital, Huazhong University of Science and Technology, Wuhan Hubei 430077, China and Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology,Wuhan Hubei 430030, China |
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| Abstract: | |
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| Keywords: | Lipoxin A4 (LXA4) Hepatocellular carcinoma (HCC) Hypoxia Proliferation |
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