温阳消饮法对胸腔积液大鼠小肠水通道蛋白4表达的影响

目的：研究温阳消饮法对胸腔积液模型大鼠小肠水通道蛋白4（AQP4）表达的影响。方法：采用计算机随机分组方法，将SD成年雄性大鼠分为温阳消饮组、模型对照组、空白对照组，每组12只。对除空白对照组外的其余2组动物以1％角叉菜胶胸腔注射建立大鼠胸腔积液（悬饮）模型，造模24h后拍X线片对模型进行病理评价。于造模成功后2h各组予相对应药物灌胃，每天1次，连续4d。灌胃结束后处死各组犬鼠，采集小肠组织，用免疫组化法检检AQP4的表达，比较各组结果。结果：与空白对照组比较，模型对照组表达降低（P〈0．05）；温阳消饮组高于模型对照组（P〈0．05），与空白对照组比较差异无统计学意义（P〉0．05）。结论：温阳消饮法可上调胸腔积液模型大鼠小肠AQP4的表达。

Effect of yang - warming and fluid retention - resolving therapy on expression of aquaporin - 4 in small intestine among rats with pleurai effusion

Objective ： To study the effe,t of yang - warming and fluid retention - resolving therapy （YWFRRT） on the expression of aquaporin -4 （AQP4） in the small intestine among rats with pleural effusion. Methods：Aduh male Sprague -Dawley rats were digital- ly randomized into YWFRRT group （ n = 12 ）, model control group （ n = 12 ）, and blank control group （ n = 12 ）. All rats except those in blank eontrol group received intrapleural injection of 1% carrageenan to establish a rat model of pleural effusion （ pleural fluid reten- tion）. At ：24 h after the model was established, model rats were subjected to pathological evaluation by radiography. At 2 h after the model was established, each group received respective treatments by garage once daily for 4 d. All rats were sacrificed after intragastric administration,and small intestine tissues were collected. The expression of AQP4 was measured by immunohistochemistry,and the re- suits were compared between groups. Resuhs：Compared with the blank control group,the model control group showed significant de- crease in AQP4 expression（P 〈 0. 05 ） ;the YWFRRT group had significantly higher AQP4 expression than the model control group （ P 〈 0. 05 ） , but there was no significant difference between the YWFRRT group and blank control group （ P 〉 0. 05 ）. Conclusion ： YWFRRT can up - regulate AQP4 expression in the small intestine among mice with pleural eflusion.