针刺经穴特异性对小鼠耐缺氧时间影响的研究

目的：观察针刺不同经穴、非经穴对小鼠耐缺氧时间的影响，进一步证实经穴特异性的客观存在，优化临床选穴。方法：将168只体质量相同的小鼠随机分为经穴组和非经穴组，经穴组又分为足三里组和三阴交组，非经穴组分为鼠尾组和空白组，每组各42只。用固定器固定小鼠，足三里组、三阴交组、鼠尾组分别予以针刺治疗，施平补平泻手法5min，留针20min；空白组予以相同的时间固定而不进行针刺治疗，观察比较4组小鼠的耐缺氧时间。结果：足三里组小鼠的耐缺氧时间最长，足三里组、三阴交组、鼠尾组较空白组小鼠的耐缺氧时间延长，差异有统计学意义（P〈0．05）；足三里组、三阴交组较鼠尾组小鼠的耐缺氧时间长，差异有统计学意义（P〈0．05）；足三里组较三阴交组小鼠的耐缺氧时间长，但差异无统计学意义（P〉0．05）。结论：针刺经穴能显著提高小鼠的耐缺氧时间，足三里穴效果最显著，非经穴作用明显低于经穴组，经穴特异性客观存在。

Effect of acupuncture at specific acupoints on hypoxia tolerance time in mice

Objective ：To observe the effects of acupuncture at different acupoints and non -acupoint acupuncture on the hypoxia tolerance time in mice and to confirm the existence of acnpoint specificity and optimize clinical acupoint selection. Methods：A total of 168 mice with the same body weight were randomly divided into acupoint group and non- acupoint group ;the acupoint group was fur- ther divided into Zusanli group （ n = 42） and Sanyinjiao group （ n = 42）, and the non - acupoint group was further divided into mouse tail group （n = 42） and blank group （n = 42 ）. After being fixed with a fixator, the Zusanli group, Sanyinjiao group, and mouse tail group received acupuncture （neutral supplementation and draining for 5 min and needle retaining for 20 min） ;the blank group was fixed for the same length of time without acupuncture. The four groups were compared in terms of hypoxia tolerance time. Results：The Zusanli group had the longest hypoxia tolerance time, and the Zusanli group, Sanyinjiao group, and mouse tail group had a significantly prolonged hypoxia tolerance time compared with the blank group （P 〈 0. 05） ;the Zusanli group and Sanyinjiao group had a significantly longer hypoxia tolerance time than the mouse tail group （P 〈 0. 05） ;the Zusanli group had a nonsignificantly longer hypoxia tolerance time than the Sanyinjiao group （ P 〉 0. 05 ）. Conclusion ： Acupoint acupuncture can significantly prolong the hypoxia tolerance time in mice and has the best efficacy when applied at the Zusanli acupoint. Non - acupoint acupuncture has lower efficacy than acupoint acu- puncture, suggesting the existence of acupoint specificity.