| HPLC法测定金英胶囊中盐酸小檗碱的含量 |
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| 引用本文： | 彭艳梅,谢谊,刘会,陈波. HPLC法测定金英胶囊中盐酸小檗碱的含量[J]. 湖南中医杂志, 2014, 30(2): 129-130 |
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| 作者姓名： | 彭艳梅 谢谊 刘会 陈波 |
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| 作者单位： | 彭艳梅(湖南省中医药研究院中药研究所,湖南长沙,410013)；谢谊(湖南省中医药研究院中药研究所,湖南长沙,410013)； 刘会 (湖南省中医药研究院附属医院,湖南长沙,410006)； 陈波 (湖南方盛制药有限公司,湖南长沙,410013)； |
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| 摘 要： | 目的：建立HPLC法测定金英胶囊中黄柏的总盐酸小檗碱含量。方法：采用HypersilBDS—C-18（250mm×4．6mm，5μm）色谱柱，0．05mol／L磷酸二氢钾溶液一乙腈（78：22）为流动相，检测波长为346nm，流速为1．0mL／min。理论板数按盐酸小檗碱计算应不低于3500。结果：本品每粒含黄柏以盐酸小檗碱计，不得少于1．0mg。结论：该方法操作简单，重现性好，适用于金英胶囊的质量控制。
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| 关 键 词： | HPLC 金英胶囊 盐酸小檗碱 含量测定 |
Determination of berberine hydrochloride in Jinying capsules by high - performance liquid chromatography |
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| PENG Yan- mei,XIE Yi,LIU Hui,CHEN Bo. Determination of berberine hydrochloride in Jinying capsules by high - performance liquid chromatography[J]. Hunan Journal of Traditional Chinese Medicine, 2014, 30(2): 129-130 |
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| Authors: | PENG Yan- mei XIE Yi LIU Hui CHEN Bo |
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| Affiliation: | 1. Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha 410013, Hunan, China; 2. The Affiliated Hospital of Hunan Academy of Chinese Medicine, Changsha 410006, Hunan, China; 3. Hunan Fangsheng Pharmaceutical Co. ,Ltd. ,Changsha 410013 ,Hunan,China) |
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| Abstract: | Objective： To establish a method for determining the total berberine hydrochloride content of Cortex Phellodendri Chinensis in Jinying capsules by high - performance liquid chromatography （HPLC）. Methods ： HPLC was performed on a Hypersil BDS -C-18 Scolumn （250 mm ×4. 6 mm,5 μm） with a mobile phase of 0.05 mol/L monopotassium phosphate- acetonitrile （78： 22） at a detection wavelength of 346 nm and a flow rate of 1.0 ml/min. The number of theoretical plates would be not less than 3500, as calcu- lated by berberine hydrochloride. Results ：The content of Cortex Phellodendri Chinensis in each Jinying capsule, as calculated by berber- ine hydrochloride,would be not less than 1.0 mg. Conclusion ：The HPLC method has the advantages of easy operation and good repeat- ability and is suitable for the quality control of Jinying capsules. |
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| Keywords: | high - performance liquid chromatography Jinying capsule berberine hydrochloride determination |
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