小鼠胚泡黏附时子宫内膜Galectin-1的变化

目的：探讨小鼠胚胎黏附时子宫内膜Galectin-1的变化。方法：双向聚丙烯酰胺凝胶电泳(2D-PAGE)分析妊娠d5小鼠胚泡黏附时着床点、着床旁子宫内膜总蛋白，以同龄未交配鼠子宫内膜为对照，差异蛋白质组学显示pⅠ约5．1、分子量约15ku的蛋白点在着床点、着床旁子宫内膜表达上调，且在着床点更明显。对此蛋白点用基质辅助激光解析电离飞行时间质谱测定其胶内酶解后的肽质量指纹谱(peptidemassfingerprint，PMF)。结果：Mascot：PMF在SWISS．PROT数据库查询为鼠源性Galectin．1。RT．PCR．~果显示d5孕小鼠子宫内膜Galectin．1mRNA水平也明显增加。结论：Galectil9．1可能参与胚泡着床这一重要生命活动过程。

Expression of Galectin-1 in Mice Endometrium when Blastocysts Hatched into the Endometrium

Objective: To observe the expression of galectin-1 in the endometrium when blastocysts hatched.Method: Protein expression patterns of mice blastocyst implantation sites and peri-implantation sites from d 5 ofpregnancy were analyzed by 2D gel electrophoresis and differential proteomics,with the virgin mouse as the control.Result: A protein with pI 5.1, MW 15 ku showed up-regulated expression in uteri at d 5 with matrix-assisted laserdesorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS), this protein was identified as Galectin-1(LEG1_MOUSE). The expression of Galectin-1 mRNA was significantly different among the three groups by RT-PCR. Conclusion: The data suggested that Galectin-1 was possibly involved in the blastocyst implantation.