Androgen rather than estrogen up-regulates brain-type cytochrome P450 aromatase (cyp19a1b) gene via tissue-specific promoters in the hermaphrodite teleost ricefield eel Monopterus albus |
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Authors: | Zhang Yang Zhang Shen Zhou Wenliang Ye Xing Ge Wei Cheng Christopher H K Lin Haoran Zhang Weimin Zhang Lihong |
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Affiliation: | a Department of Biology, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China b State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China c Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, PR China d Department of Biology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong e School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong |
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Abstract: | CYP19A1 in the brain and pituitary of vertebrates is important for reproductive and non-reproductive processes. In teleosts, it is broadly accepted that estradiol (E2) up-regulates cyp19a1b gene via a positive autoregulatory loop. Our present study, however, showed that E2 did not up-regulate ricefield eel cyp19a1b in the hypothalamus and pituitary, whereas dihydrotestosterone (DHT) or testosterone (T) stimulated cyp19a1b expression only in the pituitary. Two tissue-specific promoters, namely promoter I and II directing the expression in the brain and pituitary respectively, were identified. Promoter I contained a non-consensus estrogen response element (ERE), and consequently did not respond to E2. Promoter II contained an androgen response element (ARE) and consequently responded to DHT. Taken together, these results demonstrated a novel steroidal regulation of cyp19a1b gene expression and an alternative usage of tissue-specific cyp19a1b promoters in the brain and pituitary of a teleost species, the ricefield eel. |
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Keywords: | Monopterus albus cyp19a1b Tissue-specific promoter Estrogen Androgen |
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