实时细胞分析结合指纹图谱技术比较不同部位雷公藤生物活性

目的基于实时细胞分析技术结合HPLC法,比较不同部位、不同产地雷公藤的生物活性。方法建立x CELLigence实时细胞分析系统实时动态检测细胞活性的方法:接种密度为2×105/m L,给药时间为24 h左右,检测雷公藤对RBL-2H3细胞的活性;通过HPLC法建立不同部位雷公藤的指纹图谱并分析其共有峰。结果浙江产雷公藤同一部位活性相似;根皮和叶具有类似生物学活性,根和茎具有类似的活性,根皮、嫩芽对RBL-2H3细胞的生物活性明显高于根、茎部位,尤以根皮部活性最强;指纹图谱显示不同部位成分相似但量差异较大;氯仿提取液对RBL-2H3细胞的抑制率明显高于水提液。结论在细胞生物学层面上雷公藤根皮及地上部分有研究与药用价值,可对其深入开发和利用。

Comparison of cell activity from different parts of Tripterygium wilfordill based on xCELLigence system

Objective To compare the bio-activities of different parts of Tripterygium wilfordii from different regions based on RTCA system with HPLC. Methods Real-time xCELLigence analysis system was established to detect dynamically cellular activity at real-time:The seeding density was 2×10⁵ cells/mL, the administration time was about 24 h, and the activity of RBL-2H3 cells from T. wilfordii was detected; The fingerprints of different parts of T. wilfordii were established by HPLC and the common peaks were analyzed. Results The activity of T. wilfordii from Zhejiang was similar at the same site; The root barks and leaves of T. wilfordii had similar biological activity, and the roots and stems had similar activity; The biological activity for RBL-2H3 cells in root barks and buds was significantly higher than that in roots and stems, especially in the root barks, biological activity was the strongest; The fingerprints showed that the components of different parts were similar but the contents were largely different; The inhibitory rate of chloroform extract on RBL-2H3 cells was significantly higher than that of water extract. Conclusion At the level of cell biology, the velamen of T. wilfordii and other aerial parts possess value of research and medication, which is deserved to further developed and utilized.