红花DHDPS基因全长cDNA的克隆及植物表达载体构建 Cloning of full-length cDNA of gene encoding dihydrodipicolinate synthase from <i>Carthamus tinctorius</i> and construction of plant expression vector期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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红花DHDPS基因全长cDNA的克隆及植物表达载体构建

引用本文：	王艳芳,张玲,韩红祥,于洋,臧埔,毛欣欣,马吉胜,买地努尔&#;买合木提,方阔,丁玲,杨小萍,蒋杰,雷锋杰,包京姗,王壮,尹春梅,李海燕,李校堃.红花DHDPS基因全长cDNA的克隆及植物表达载体构建[J].中草药,2017,48(8):1629-1634.

作者姓名：	王艳芳张玲韩红祥于洋臧埔毛欣欣马吉胜买地努尔&#;买合木提方阔丁玲杨小萍蒋杰雷锋杰包京姗王壮尹春梅李海燕李校堃

作者单位：	吉林农业大学中药材学院, 吉林长春 130118;吉林农业大学生物反应器与药物开发教育部工程研究中心, 吉林长春 130118;吉林农业大学中药材学院, 吉林长春 130118;吉林农业大学生物反应器与药物开发教育部工程研究中心, 吉林长春 130118;吉林农业大学中药材学院, 吉林长春 130118;吉林农业大学科技管理处, 吉林长春 130118;吉林农业大学中药材学院, 吉林长春 130118;吉林农业大学中药材学院, 吉林长春 130118;吉林农业大学中药材学院, 吉林长春 130118;吉林农业大学生物反应器与药物开发教育部工程研究中心, 吉林长春 130118;吉林农业大学生命科学学院, 吉林长春 130118;吉林农业大学中药材学院, 吉林长春 130118;吉林农业大学中药材学院, 吉林长春 130118;吉林农业大学中药材学院, 吉林长春 130118;吉林农业大学中药材学院, 吉林长春 130118;吉林农业大学中药材学院, 吉林长春 130118;吉林农业大学中药材学院, 吉林长春 130118;吉林农业大学中药材学院, 吉林长春 130118;吉林农业大学中药材学院, 吉林长春 130118;吉林农业大学生物反应器与药物开发教育部工程研究中心, 吉林长春 130118;吉林农业大学生命科学学院, 吉林长春 130118;吉林农业大学中药材学院, 吉林长春 130118;吉林农业大学生物反应器与药物开发教育部工程研究中心, 吉林长春 130118;吉林农业大学生命科学学院, 吉林长春 130118

基金项目：	国家自然科学基金资助项目（31401445）；吉林省教育厅“十三五”科学技术项目（JJKH20170310KJ）；吉林省科技厅医药产业推进计划项目（20140311034YY）；国家高技术研究发展计划（“863”）项目（2011AA100606）；2017年吉林农业大学大学生创新创业训练计划项目

摘要：	目的克隆红花赖氨酸生物合成途径关键酶二氢吡啶二羧酸合酶(dihydrodipicolinate synthase DHDPS)基因的全长c DNA序列,构建植物超表达载体。方法根据红花转录组文库注释信息获得红花DHDPS(CtDHDPS)基因的中间序列,通过RT-PCR与RACE技术从红花种子中克隆CtDHDPS基因全长c DNA序列。采用DNA重组技术构建p BASTA-CtDHDPS植物超表达载体。结果分离CtDHDPS基因全长1 309 bp,开放阅读框954 bp,编码317个氨基酸,编码蛋白理论等电点为5.93,相对分子质量约为34 750.79。通过DNA重组技术成功构建了植物超表达载体p BASTA-CtDHDPS。结论获得CtDHDPS基因全长c DNA序列并成功构建植物超表达载体,为阐明CtDHDPS基因的生物学功能及其在红花赖氨酸生物合成途径中作用机制提供科学依据。
关键词：	红花二氢吡啶二羧酸合酶 RACE 生物信息学分析植物表达载体
收稿时间：	2016/11/13 0:00:00
*Cloning of full-length cDNA of gene encoding dihydrodipicolinate synthase from Carthamus tinctorius* and construction of plant expression vector**

WANG Yan-fang,ZHANG Ling,HAN Hong-xiang,YU Yang,ZANG Pu,MA Ji-sheng,MAO Xin-xin,MAI Dinuer&#;MAI Hemuti,FANG Kuo,DING Ling,YANG Xiao-ping,JIANG Jie,LEI Feng-jie,BAO Jing-shan,WANG Zhuang,YIN Chun-mei,LI Hai-yan and LI Xiao-kun.Cloning of full-length cDNA of gene encoding dihydrodipicolinate synthase from Carthamus tinctorius and construction of plant expression vector[J].Chinese Traditional and Herbal Drugs,2017,48(8):1629-1634.

Authors:	WANG Yan-fang ZHANG Ling HAN Hong-xiang YU Yang ZANG Pu MA Ji-sheng MAO Xin-xin MAI Dinuer&#;MAI Hemuti FANG Kuo DING Ling YANG Xiao-ping JIANG Jie LEI Feng-jie BAO Jing-shan WANG Zhuang YIN Chun-mei LI Hai-yan and LI Xiao-kun

Institution:	Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;College of Life Sciences, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;College of Life Sciences, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;Department of Science and Technology Management, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;College of Life Sciences, Jilin Agricultural University, Changchun 130118, China;College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;College of Life Sciences, Jilin Agricultural University, Changchun 130118, China;College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;College of Life Sciences, Jilin Agricultural University, Changchun 130118, China;College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, China

Abstract:

Keywords:	Carthamus tinctorius L DHDPS RACE bioinformatics analysis plant expression vector
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