山药提取物联合DC-CIK细胞疗法对结肠癌HT29干细胞荷瘤裸鼠的体内抗肿瘤研究

目的观察山药提取物联合树突状细胞-细胞因子诱导的杀伤细胞(DC-CIK)疗法对结肠癌HT29细胞干细胞荷瘤裸鼠的治疗效果。方法建立Balb/c裸鼠结肠癌干细胞荷瘤模型,随机分为4组,对照组、DC-CIK组、山药提取物组、山药提取物联合DC-CIK组(联合治疗组),每组10只。其中DC-CIK组、联合治疗组裸鼠在肿瘤干细胞接种第4天后通过尾iv 1×106个DC-CIK细胞给予治疗,每周2次,共3周;山药提取物组和联合治疗组,按山药提取物125 mg/kg给药,每天ig给药1次,共3周;对照组以等体积生理盐水代替。各组裸鼠在治疗3周期间每2天测量瘤体大小及裸鼠体质量,治疗结束后处死裸鼠,取出瘤体称质量,并计算抑瘤率,RT-PCR法检测瘤组织中信号通路关键基因的表达水平。结果治疗结束后,山药提取物组、DC-CIK组、联合治疗组的瘤体质量明显低于对照组,其中,联合治疗组抑瘤率为51.26%。在信号通路关键基因的表达水平变化方面,联合治疗组与DC-CIK组、山药提取物组相比,PI3K/Akt通路中关键基因PI3KR1,Wnt/β-catenin通路中关键基因Wnt1,Notch通路中关键基因Notch1的m RNA表达均有所下调,DC-CIK组和山药提取物组相比,各基因m RNA表达变化差异不显著。结论对结肠癌HT29细胞干细胞荷瘤裸鼠模型,山药提取物联合DC-CIK抑瘤效果最佳。

In vivo antitumor effect of Chinese yam extract combined with DC-CIK on nude mice bearing colon cancer HT29 stem cells

Objective To compare the therapeutic effects of different treatments on the nude mice bearing colon cancer HT29 cells. Methods BalB/C nude mice colon cancer stem cell model was established and the mice were randomly divided into following four groups:blank control group, DC-CIK group, yam group, and yam combined with DC-CIK group (combined treatment group), each group of 10 nude mice. In DC-CIK group and combined treatment group, after tumor stem cells were inoculated in nude mice for 4 d, 1×10⁶ DC-CIK cells were used through the tail vein injection for treatment, twice a week for three weeks. The mice in Chinese yam group and combined treatment group were ig administered with 125 mg/kg Chineseyam extract, once daily for three weeks, and the mice in control group were treated with equal volume of saline instead. Tumor size and body weight of nude mice were measured every 2 d during treatment for three weeks in each group. After the treatment, the nude mice were sacrificed to take out the tumor weight and the tumor inhibitory rate was calculated. The RT-PCR method was used to detect the expression levels of the key genes in signal pathway. Results After treatment, the quality of tumor in yam group, DC-CIK group and combined treatment group was significantly lower than that in control group. Among them, the inhibitory rate was 51.26% in combined treatment group. In the changes of expression levels of key genes in signaling pathway, the key genes of PI3KR1 in PI3K/Akt pathway, the key gene of Wnt1 in Wnt/beta-catenin pathway, and the mRNA expression of key gene Notch1 in Notch pathway in combined treatment group were decreased compared with DC-CIK group and yam group. Compared with the DC-CIK group, the mRNA expression changes were not statistically significant. Conclusion The effect of Chinese yam combined with DC-CIK group is the best on the treatment of HT29 cell stem cell tumor of colon cancer in nude mice. It provides a new idea for clinical treatment of colon cancer.