| Regularity of ultraviolet (UV)-induced NIH3T3 cell damage and characteristics of DNA cleavage in UV-induced NIH3T3 cells apoptosis |
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| 作者姓名： | 胡庆柳 丁振华 |
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| 作者单位： | Department of Radiation Medicine，Faculty of Preclimcal Medicine，First Military Medical University，Guangzhou 510515，Guangdong Province，China |
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| 摘 要： | INTRODUCTIONThesolarspectrumonthesurfaceoftheearthcomprisesultraviolet(UV),visibleandinfraredradiation,withrelativeintensityof3%,37%and60%respectively.Inaddition,X--raysandradiowaveshavebeendetected,however,theirintensityisnegligiblylowllj.AlthoughUVradiationisaminorpartofthesolarspectrum,itisconsideredtobeveryeffective.Basedondifferencesinbiologicalresponses,UVradiationissubdividedintothreewavebands:UVC(100urn--280"m),UVB(180urn--320urn)andUVA(320urn--400"m)ZJ.Inthisstudy,cytotox…
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Regularity of ultraviolet (UV)-induced NIH3T3 cell damage and char acteristics of DNA cleavage in UV-induced NIH3T3 cells apoptosis |
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| Hu Qingliu, Ding Zhenhua.Regularity of ultraviolet (UV)-induced NIH3T3 cell damage and characteristics of DNA cleavage in UV-induced NIH3T3 cells apoptosis[J].Journal of Medical Colleges of PLA(China),1998(4). |
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| Authors: | Hu Qingliu Ding Zhenhua |
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| Abstract: | To study the regularity of Ultraviolet (UV)-induced NIH3T3 cell damage and characteristics of DNA cleavage in UV-induced NIH3T3 cells apoptosis. Methods: Regularity of ultraviolet (UV)induced NIH3T3 cell damage was studied by two ways: The cells were irradiated for different time lengths and cultured for the same time (24 h) length and irradiated for same time length (10 min), cultured for different time length. The characteristics of DNA cleavage in NIH3T3 cells apoptosis induced by UVB were also studied. Results: It was found that ultraviolet A (UVA), ultraviolet B (UVB), ultraviolet C (UVC) have their particular regular patterns of cell damage respectively. Conclusion: DNA cleavage of apoptotic cells does not always initiate at inter-nucleosomes. Inter-nucleosomal DNA cleavage should not be the sole criterion for identifying apoptosis. |
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| Keywords: | UV irradiation NIH3T3 apoptosis |
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