荧光定量PCR测定水杨酸钠作用后大鼠耳蜗基因的表达

目的了解HSP27基因在大剂量水杨酸钠作用后大鼠耳蜗中表达水平，并探讨其在水杨酸钠耳毒性中的意义。方法将Wistar大鼠各15只分成水杨酸钠作用组和正常对照组，应用SYBR GREEN I实时监测的逆转录-聚合酶链反应(RT-PCR)，检测大鼠耳蜗中HSP27基因在长期大剂量水杨酸钠作用后和正常对照组中的表达,并用管家基因β actin作为内参。结果水杨酸钠作用后大鼠耳蜗中HSP27基因的表达水平高于正常对照组，差异有统计学意义(P＜0.01)。结论SYBR GREEN I定量PCR法可以作为一种良好的方法对来源珍贵的微量组织的基因表达进行检测, 水杨酸钠能够明显诱导HSP27基因在大鼠耳蜗中的表达。

Expression of the heat shock protein 27 in rat cochlea induced by sodium salicylate injection by the FQ-PCR technique

To investigate the gene expression of HSP27 in the cochlea of rats induced by sodium salicylate injection and to explore its role in sodium salicylate ototoxity. MethodsThe HSP27 gene expression in specimens was determined by using real time RT-PCR with SYBR GREEN I, and β-actin served as the internalcontrol. ResultsThe gene expression of HSP27 in the cochlea of rats induced by sodium salicylate injection was significantly increased compared with normal rats(P＜0.01). ConclusionQuantitative PCR with SYBR GREEN I fluorescence is a reliable method to determine the gene expression of rarely available tissues. Sodium salicylate has an effect on inducing the gene expression of HSP27 in the cochlea of rats.