血红素氧化酶-1基因启动子区（GT）n重复序列多态性与原发性高血压及冠心病易感性的关联研究

目的：探讨HO-1基因启动子区（GT）n重复序列多态性与原发性高血压、冠心病以及原发性高血压合并冠心病易感性间的关联。方法：采用荧光标记PCR以及毛细管电泳相结合技术,检测HO-1基因启动子区（GT）n重复序列多态性在91例原发性高血压、111例冠心病、189例原发性高血压合并冠心病患者,以及性别、年龄相匹配的91例对照个体中等位基因和基因型频率分布差异。多因素降维法分析基因型与吸烟、糖尿病、高血脂等危险因素间的交互作用。结果：与对照组比,等位基因频率仅在冠心病组中分布有统计学意义（P〈0.05）,SL＋LL基因型频率在原发性高血压（P〈0.05）和冠心病组（P〈0.01）中有统计学意义（P〈0.05）,而在原发性高血压合并冠心病组中无统计学意义。SL＋LL基因型与吸烟交互作用增加了原发性高血压和冠心病的发病风险,而对于原发性高血压合并冠心病发病风险无影响。结论：HO-1基因启动子区（GT）n重复序列多态性可作为原发性高血压、冠心病发病风险评估的分子遗传标记。

Association Study of (GT)n Polymorphism in Hemeoxygenase-1 Gene Promoter and Susceptibility of Primary Hypertension and Coronary Artery Disease

Objective:To assess the association between the length of(GT)n repeats in the HO-1 gene promoter and the susceptibility for patients of primary hypertension,coronary artery disease,primary hypertension with coronary artery disease.Methods:Amplification and labeling of PCR products for capillary electrophoresis were performed to genotype.Ninety-one patients with primary hypertension,111 patients with coronary artery disease,189 patients of primary hypertension with coronary artery disease and 91 control subj...