P53蛋白在胰腺癌与癌旁组织中表达的临床意义

目的：了解胰腺癌及其癌旁组织的ｐ５３蛋白表达，并分析其临床意义。方法：作者对３６例胰腺导管癌、２５例胰腺癌旁组织、１０例慢性胰腺炎和１２例正常胰腺组织石蜡标本进行ｐ５３单抗的免疫组化染色。结果：３６例胰腺癌和２５例癌旁组织中呈ｐ５３蛋白阳性染色分别为２１例（５８％）和１１例（４４％），且其阳性率间有显著相关性。（Ｐ＜０．０１）。而慢性胰腺炎和正常胰腺组织均未见ｐ５３蛋白阳性染色，与胰腺癌和癌旁组织ｐ５３阳性率间均有显著差异（Ｐ＜０．０１）。不同临床分期及组织学分型胰腺癌，ｐ５３蛋白阳性染色率间无显著差异。结论：ｐ５３蛋白的表达与胰腺癌的发生有关；胰腺癌旁组织中某些细胞呈ｐ５３阳性染色，这些细胞可能较易发生癌变，使胰腺癌呈多中心性发生。

Immunohistochemical analysis of p53 expression in pancreatic carcinomas and adjacent tissues

Purpose: In order to explore the expression of p53 protein in pancreatic adenocarcinomas and their adjacent tissues. Methods: We used immunohistochemical method to examine formalin-fixed paraffinembedded samples of 36 pancreatic adenocarcinomas, 25 nommalignant peri-tumoral tissues, 10 chronic pancreatitis and 12 normal pancreatic tissues for overexpression of the p53 gene product. Results: Specific nuclear staining for the p53 protein was identified in 21 out of 36 carcinomas and 11 out of 25 nonmalignant peri-tumoral tissues. The positive rate of p53 was significantly higher in cancer or adjacent tissues than chronic pancreatitis or normal panreatic tissues (P<0.01). Besides, no significant difference of p53 staining was found among different clinical stages and histologic differentiation. Conclusion: p53 protein is involved in the carcinogenesis if pancreatic adenocarcinomas. In addition, the nonmalignant peritumoral tissues staining positively for p53 protein might reveal their susceptibility of tumorogenesis.