大蹼铃蟾抗菌肽-2对膀胱癌细胞株HLA-ABC以及DR表达影响的对照实验研究

目的　探讨大蹼铃蟾抗菌肽 2 (Maximin 2 )对膀胱癌细胞株HLA DR及HLA ABC表达的影响 ,并与TNF α、IFN α进行比较。方法　采用肿瘤细胞培养方法 ,流式细胞仪检测各实验样品对膀胱癌细胞株T2 4、BIU 87、SCaBER的HLA DR及HLA ABC表达的影响。结果　Maximin 2对不同的膀胱癌细胞株在小剂量下即有抑制作用 ,并呈剂量相关性 ,各实验样品未见对各膀胱癌细胞株的HLA DR表达有影响 ,Maximin 2、TNF α对HLA ABC的表达均无影响 ;IFN α则对HLA ABC的表达有上调作用。结论　Maximin 2、TNF α抗癌机制与提高肿瘤细胞HLA DR、ABC的表达无关 ,IFN α可通过提高肿瘤细胞HLA ABC的表达提高T细胞对膀胱肿瘤的识别杀伤能力

The comparative experiment of maximin-2 on expression of HLA-DR HLA-ABC by cultured bladder cancer cell lines

AIM To study the boosting effect of Maximin 2 on expression of HLA DR and HLA ABC by cultured bladder cancer cell lines and compare with TNF α?IFN α. METHODS Cell culture and FCM were used to dectect the effects of Maximin 2,TNF and IFN on the cell growth of three types of bladder cancer cell lines and HLA DR and HLA ABC expression under the IC 50 culture concentration. RESULTS Maximin 2 had show inhibition to cells growth under the small dosage. TNF α?IFN α also had the effect on restrain all 3 cell lines growth. The expression of HLA DR was not any change in all 3 cell lines after being stimulated by Maximin 2?TNF α and IFN α, Maximin 2 and TNF α can not change HLA ABC expression, IFN α can up regulate HLA ABC expression. CONCLUSION Maximin 2 show the dose dependent effect on cell growth suppression; The mechanism of antitumor of Maximin 2 seems not relate to the enhanced expression of HLA DR or HLA ABC, TNF α and IFN α also not found effect to HLA DR expression. Since IFN α are able to increase the expression of HLA ABC expression,enhanced recognization and cytotoxity of CTL to bladder cancer cells. It is may be one of the mechanism of antitumor of IFN α.