| 人博卡病毒LAMP检测方法的建立及初步应用 |
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| 引用本文： | 王翔,许信刚,张骞,张钫,袁武梅,麻粉莲,薛鹏浩,郑丽舒. 人博卡病毒LAMP检测方法的建立及初步应用[J]. 中华实验和临床病毒学杂志, 2010, 24(4): 308-310. DOI: 10.3760/cma.j.issn.1003-9279.2010.04.023 |
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| 作者姓名： | 王翔 许信刚 张骞 张钫 袁武梅 麻粉莲 薛鹏浩 郑丽舒 |
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| 作者单位： | 1. 西北农林科技大学动物医学院,712100
2. 中国疾病预防控制中心病毒病预防控制所病毒基因工程国家重点实验室 |
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| 基金项目： | 传染病防治科技重大专项 |
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| 摘 要： | 目的 建立环介导等温扩增技术（LAMP）检测人博卡病毒（Human bocavirus,HBoV）基因的方法,并用于检测临床样本.方法 通过在线软件设计工具Ptimer Explorer V4设计HBoV NP-1基因的LAMP引物,建立LAMP检测方法,并评价其灵敏度和特异度.结果 与传统PCR方法相比,LAMP法检测灵敏度更高,可达到10拷贝,特异性较强;在76份呼吸道感染儿童的咽拭子标本中检测到5份阳性.结论 建立HBoV LAMP检测方法灵敏特异,简单快速,有望进行临床检测HBoV.
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| 关 键 词： | 细小病毒科 基因 核酸扩增技术 |
Establish and preliminary application of loop mediated isothermal amplification (LAMP) technique in detection for human bocavirus |
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| WANG Xiang,XU Xin-gang,ZHANG Qian,ZHANG Fang,YUAN Wu-mei,MA Fen-lian,XUE Peng-hao,ZHENG Li-shu. Establish and preliminary application of loop mediated isothermal amplification (LAMP) technique in detection for human bocavirus[J]. Chinese journal of experimental and clinical virology, 2010, 24(4): 308-310. DOI: 10.3760/cma.j.issn.1003-9279.2010.04.023 |
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| Authors: | WANG Xiang XU Xin-gang ZHANG Qian ZHANG Fang YUAN Wu-mei MA Fen-lian XUE Peng-hao ZHENG Li-shu |
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| Affiliation: | . ( College of Veterinary Medicine, Northwest A＆F University, Yangling , Shanxi 712100,China) |
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| Abstract: | Objective To establish the methods of LAMP assay in human bocavirus(HBoV) and use to detect clinical samples. Methods Establish LAMP assay for HBoV by using primer explorer V4 (LAMP primer design software) on line to design LAMP primer for sequence NP1 of HBoV. Evaluate its sensitivity and specificity. Results Comparing with traditional PCR, LAMP shows good specificity and higher sensitivity until 10 copy. By LAMP assay we get 5 positive samples in 76 clinical swabs from the children with respiratory infections. Conclusion LAMP assay for detection of HBoV is sensitive, efficient,rapid and simple. It is a new way to detect HBoV in clinical test. |
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| Keywords: | Parvoviridae Genes Nucleic acid amplification techniques |
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