结肠腺癌组织中DNA结合/分化抑制蛋白2的表达特征及与细胞增殖的关系

目的:分析结肠腺癌组织中DNA结合/分化抑制蛋白2(inhibitor of DNA binding2，ID-2)与细胞增殖的关系。方法:收集2014年11月至2015年9月华北理工大学附属医院确诊的67例结肠腺癌患者临床资料进行回顾性分析，均行根治术，以肿瘤组织作为观察组，以距肿物边缘>3 cm处的正常结肠黏膜组织作...

Expression of DNA binding/differentiation inhibitory protein 2 in colon adenocarcinoma and its relationship with cell proliferation

Objective To detect the expression of inhibitor of DNA binding-2(ID-2)in colorectal adenocarcinoma and analyze its relationship with proliferation.Methods A total of 67 patients with colorectal adenocarcinoma diagnosed in North China University of Science and Technology Affiliated Hospital were selected from November 2014 to September 2015,tumor tissue as the research objects.All patients underwent radical operation.The tumor tissue was taken as the observation group,and the normal colonic mucosa tissue>3 cm from the edge of the tumor was taken as the control group.Immunohistochemistry was used to detect the expression of ID-2 of the two groups and the Ki67 of cancer tissue.SW480 cell line of ID-2 overexpression was constructed.The expression of proliferating cell nuclear antigen(PCNA)was detected by Western Blot.Cell activity was detected by CCK-8 test.Correlation was analyzed between ID-2 and Ki67 by pearman correlation analysis.Prognostic value of ID-2 was analyzed by Kaplan-Meier survival analysis and Log-rank test in colorectal adenocarcinoma.Results The positive rate of ID-2 was 49.3%(33/67)in the observation group,which was higher than 9.0%(6/67)in the control group,and the difference between the two groups was was statistically significant(χ²=23.927,P<0.05).In the observation group,the expression of ID-2 was statistically significant in different invasion depth(serosa and extraserosa was 68.7%(22/32),serosa and extraserosa was 31.4%(11/35)),degree of differentiation(low differentiation was 80.0%(8/10),medium differentiation was 57.9%(11/19),high differentiation was 36.8%(14/38)),clinical stage(ⅢandⅣstage were 64.3%(18/28),ⅠandⅡstage were 38.5%(15/39)),lymph node metastasis(metastasis was 70.8%(17/24),no metastasis was 37.2%(16/43))and tumor thrombus(yes was 75.0%(12/16),no was 41.2%(21/51)).The difference was statistically significant(χ²value were 6.311,4.023,4.349,6.967 and 5.575,respectively,all P<0.05).Positive correlation was found between ID-2 and Ki67(r=0.65,P<0.05).Survival analysis showed that the expression of ID-2 was related to the prognosis of patients(χ²=5.29,P=0.013).Compared with empty vector transfection group and blank control group,the expressions of PCNA and the activity ID-2 overexpression colon cancer cells increased(P<0.05).Conclusion The higher expression of ID-2 is related to clinicopathological features and prognosis in colorectal adenocarcinoma.The abnormal expression of ID-2 may play a role in regulating the proliferation of colon adenocarcinoma.