川乌、白芍治疗类风湿关节炎的网络药理学研究

目的:研究川乌、白芍治疗类风湿关节炎的作用机制.方法:以口服生物利用度(OB)≥30％、类药性(DL)≥0.18为指标,通过中药系统药理学数据库与分析平台(TCMSP)数据库分别获取川乌、白芍的活性成分、作用靶点,GeneCards数据库获取类风湿关节炎靶点,采用Cytoscape 3.7.2软件构建川乌、白芍活性成分...

Network Pharmacology of Chuanwu and Baishao in the Treatment of Rheumatoid Arthritis

Objective:To study the mechanism of Chuanwu(Radix Aconiti)and Baishao(Radix Paeoniae Alba)in the treatment of rheumatoid arthritis.Methods:According to the indexes of OB≥30%and DL≥0.18,the active components and targets of Chuanwu and Baishao were obtained by TCMSP database,and the rheumatoid arthritis targets were obtained by GeneCards database,software 3.7.2 was used to construct the protein-protein interaction(PPI)network,and finally,the mechanism of them in the prevention and treatment of rheumatoid arthritis was analyzed by Gene Ontology(GO)and Kyoto Encyclopedia of genes and genomes(KEGG)pathway enrichment.Results:A total of 97 active components of Chuanwu and Baishao were obtained,involving 1041 targets.Through screening,16 active components were obtained,and 3797 targets of rheumatoid arthritis were retrieved,including 34 targets of Chuanwu,Baishao and rheumatoid arthritis.AGE-RAGE,IL-17,TNF,Toll-like receptor,p53,FOXO,JAK-STAT,activation of P13K-Akt,nuclear factor-κB and other signaling pathways involved in KEGG.Conclusion:Chuanwu and Baishao may be related to IL-6 and protein kinase,mitogen activated protein kinase and other targets act on AGE-RAGE,IL-17,TNF,toll like receptor,p53,FOXO and other cytokines and multiple signaling pathways to play a role in the treatment of rheumatoid arthritis,suggesting that Chuanwu and Baishao have the characteristics of multi-component,multi-target and multi-channel,which is of great significance for the future study of Chuanwu and Baishao in the treatment of rheumatoid arthritis.