采用UHPLC-MS和质量亏损过滤技术分析二苯乙烯苷在大鼠体内的代谢产物和代谢途径

目的:分析二苯乙烯苷在大鼠体内的代谢产物并推测代谢途径.方法:将雄性SD大鼠随机分为血浆组(n=3)、尿液组(n=3)、胆汁组(n=3)和组织组(n=9),各组大鼠均单次灌胃二苯乙烯苷200 mg/kg,分别收集给药后10、30 min和1、1.5、2、4 h的血浆,给药后0～6 h的尿液,给药后0～4 h的胆汁以及给...

Analysis of Metabolites and Metabolism Pathway of Tetrahydroxystilbene Glucoside in Rats with UHPLC-MS and Mass Defect Filter Method

OBJECTIVE:To analyze the metabolites of tetrahydroxystilbene glucoside(THSG)and speculate its metabolism pathway in rats.METHODS:Male SD rats were randomly divided into plasma group(n=3),urine group(n=3),bile group(n=3),and tissue group(n=9).Each group was given single dose of THSG 200 mg/kg intragastrically.Plasma samples 10,30 min and 1,1.5,2,4 h after medication,the unrine 0-6 h after medication,the bile 0-4 h after medication,the tissue of heart,liver,spleen,lung,kidney and stomach 30 min and 1,2 h after medication(3 at each time point)were collected respectively.After precipitated with methanol,the metabolites of samples were analyzed and identified by UHPLC-Q-Exactive Orbitrap MS and mass loss filtration(MDF).Its metabolism pathway was speculated.RESULTS:In the blood,urine,bile,heart,liver,spleen,lung,kidney,stomach samples,6,7,11,1,5,1,3,4,4 metabolites were detected,including two phaseⅠ(hydrolysis,hydrogenation and hydroxylation)metabolites,18 phaseⅡ(glucuronic acid binding and sulfation)metabolites.There were 12 glucuronic acid binding products.CONCLUSIONS:Most of the metabolites of THSG are found in bile,mainly glucuronic acid binding products of phaseⅡmetabolite THSG;main metabolic pathways involve glucose hydrolysis,hydrogenation,hydroxylation,glucuronic acid binding and sulfation.