湿润烧伤膏对脂多糖致大鼠皮肤成纤维细胞损伤的改善作用及其机制研究

目的:研究湿润烧伤膏对脂多糖致大鼠皮肤成纤维细胞损伤的改善作用及其机制.方法:将大鼠皮肤成纤维细胞分为对照组、脂多糖组(5μg/mL)、康复新液组(阳性对照,5μg/mL脂多糖+1.25％康复新液)和湿润烧伤膏组(5μg/mL脂多糖+0.6 mg/mL湿润烧伤膏),每组设置6个复孔.以脂多糖诱导建立炎性损伤细胞摸型(对...

Study on Improvement Effect and Mechanism of MEBO on LPS-induced Injury of Rat Skin Fibroblasts

OBJECTIVE:To study the improvement effect and mechanism of MEBO on lipopolysaccharide(LPS)-induced injury of rat skin fibroblasts.METHODS:Skin fibroblasts of rats were divided into control group,LPS group(5μg/mL),Kangfuxin solution group(positive control,5μg/mL LPS+1.25%Kangfuxin solution)and MEBO group(5μg/m L LPS+0.6 mg/mL MEBO),with 6 wells in each group.Inflammatory injury cell model was induced by LPS(except for control group).After a certain period of cultivation,the cell survival rate and cell migration rate were detected in each group.The contents of TNF-αand IL-6 in cell supernatant was detected.The localization and fluorescence intensity of IL-6 protein were detected.The protein expression of PTEN,p-p65,TNF-α,IL-6,PI3 K and Akt in the fibroblasts were also determined.RESULTS:Compared with control group,survival rate of the fibroblasts was increased significantly in LPS group,while cell migration was decreased significantly;the contents of TNF-αand IL-6 in cell supernatant as well as relative protein expression of PTEN,p-p65,TNF-α,IL-6 and PI3 K were increased significantly(P<0.05 or P<0.01);IL-6 protein mainly expressed in the cytoplasm,and the fluorescence intensity was enhanced.Compared with LPS group,survival rate of the fibroblasts was decreased significantly in Kangfuxin solution group and MEBO group,while migration rate was increased significantly;the contents of TNF-αand IL-6,relative protein expression of PTEN,p-p65,TNF-α,IL-6(except for Kangfuxin solution group),PI3 K and Akt(except for Kangfuxin solution group)were decreased significantly(P<0.05 or P<0.01),while fluorescence intensity of IL-6 protein decreased;relative protein expression of TNF-α,IL-6,PI3 K and Akt in MEBO group were significantly lower than Kangfuxin solution group(P<0.05 or P<0.01).CONCLUSIONS:MEBO can inhibit the proliferation of LPS-induced skin fibroblasts,reduce the level of inflammatory factors and the intensity of inflammatory reaction,which may be related to the down-regulation of PTEN/NF-κB,PI3 K/Akt signaling pathway.