| 在大肠杆菌中高效表达人Ⅰ型免疫缺陷病毒p24蛋白 |
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| 作者单位： | ：1.解放军农牧大学研究所病毒室（金宁一 毛春生 郭志儒 殷 震）;2.河南省农业科学院畜牧兽医研究所（郭军庆） |
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| 摘 要： |
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HIGH LEVELEXPRESSION OF p24 PROTEIN OF HUMANIMMUNODEFICIENCY VIRUS TYPE 1 IN E.COLI |
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| Abstract: | In order to study thecapsid protein (p24) of human immunodeficiency virus type 1(HIV-1),a plasmid vectorpET24,containing the p24gaq gene fragment encoding the p24 protein under thecontrol of the bacteriophage T7 promoter ,was constructed.The pET24 plasmid-highlyexpressed a 30kDa protein of s10-p24 spanning 284 amino acid residues.The total amount ofthe fusion protein was approximately 38.4% of the total celluar protein in Escherchia coliBL21(DE3).Westein blotting and,immunodot blotting indicated that the recombinain proteincould be relognized by anti-p24 monoclonal antibody and HIV-1 positive serarespectively.It is significance for preparing specific antibody,and diagnostic usage. |
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