非小细胞肺癌中survivin、bcl-2和bax蛋白与多药耐药基因的表达及其相关性

目的检测非小细胞肺癌(NSCLC)中凋亡蛋白survivin、bcl2、bax与多药耐药有关基因MDR1mRNA和MRPmRNA的表达,探讨其相互关系及意义。方法选取113例NSCLC病例,采用原位分子杂交检测MDR1和MRP基因mRNA的表达,SP免疫组化法检测survivin、bcl2、bax蛋白的表达。结果113例NSCLC中,MDR1mRNA、MRPmRNA及survivin、bcl2、bax蛋白的检出率分别为51.3%、80.5%、79.6%、59.3%、54.9%。survivin和bax的表达与肿瘤分化程度有关(P<0.05),bcl2的表达与肿瘤组织学类型有关(P<0.01)。MDR1mRNA与MRPmRNA(P<0.01)、survivin与MDR1mRNA(P<0.05)、bcl2与MRPmRNA(P<0.05)表达之间存在明显相关性。结论凋亡抑制蛋白survivin的高表达和bcl2的过表达可能是导致NSCLC原发耐药的重要因素,检测survivin和bcl2对临床逆转NSCLC多药耐药性具有指导意义

The Relationship between Expression of survivin,bcl-2,bax and Multidrug Resistance Gene in Non-small Cell Lung Cancer

Objective To investigate the expression of apoptosis proteins survivin,bcl-2 ,bax and the multidrug resistance(MDR)associated genes MDR1mRNA,MRPmRNA in non-small cell lung cancer(NSCLC),their relationship and significance.Methods The expression of MDR1mRNA and MRPmRNA in 113 NSCLC specimens were detected by in situ hybridization,and the expression of survivin,bcl-2 and bax in the same specimens were detected by immunohistochemistry.Results The positive rates of 113 NSCLC samples for MDR1 mRNA,MRP mRNA,survi...