Bat SARS coronavirus HKU3-1病毒株基因组及蛋白质的生物信息学分析

目的比较中国菊头蝠SARS冠状病毒(Bat SARS coronavirus)与人类及果子狸体内分离出来的SARS-Cov,分析它们是否存在一定的相似性。方法本文分析了Bat SARS coronavirus与已知冠状病毒Human SARS-CoV和civet SARS-CoV的系统进化关系,绘制了Bat SARS coronavirusHKU3-1的全基因序列图,同时预测了S蛋白和N蛋白的三维模型。结果对Bat SARS coronavirus HKU3-1的序列分析表明中国菊头蝠SARS-CoV与人和果子狸SARS-CoV非常相近。系统进化分析法显示Bat-SARS-CoV与SARS-CoV形成一个不同的群,称为group 2b CoV,与已知的group 2 CoV存在一定的距离。结论Bat SARS coronavirus HKU3-1不太可能是从人SARS-CoV传播过来的。另外,Bat SARS coronavirus与civet SARS-CoV可能具有共同的祖先,使得中国菊头蝠SARS病毒株同样具有感染人体的危险。

Bioinfomatics analysis on genome and protein of Bat SARS coronavirus HKU3-1

[Objective] To further compare Bat SARS coronavirus with coronaviruses of human and civets, in order to explore their similarities. [Methods] A system anagenesis relation among Bat SARS coronavirus, Human SARS coronavirus, and Civet SARS coronavirus was explored in present study, and a genetic sequence map was drawn. Additionally, a 3D model of S protein and N protein was predicted. [Results] The sequence analysis showed that Bat SARS coronavirus was with highly similarity as SARS coronaviruses of human and palm civets. The system anagenesis analysis demonstrated that Bat-SARS-CoV and SARS-CoV formed a group called Group 2b CoV, which was distant to Group 2 CoV. [Conclusion] It is not very possible that Bat SARS coronavirus HKU3-1 was not transmitted from Human SARS coronavirus, but Bat SARS coronavirus and Civet SARS coronavirus may share the same origin, which implies Bat SARS coronavirus might still be infectious to human.