冰糖增加口服四环素吸收作用机理的初步研究

目的 :对中药冰糖增加口服四环素吸收作用机理进行初步的实验研究。方法 :参考 Masahiro Fukahori等介绍的方法 ,分别给大白鼠单独口服 (灌胃 )四环素或合并不同浓度冰糖溶液 (2 0 %、40 %、6 0 %、80 % )口服 (灌胃 )后 ,采用改进的荧光光度法测定血药浓度及肠内液药物浓度及含量 ,并称量与比较肠内容物量。结果 :合并使用冰糖可使大白鼠血中四环素的浓度明显提高 (P<0 .0 5 ) ,并在 2 0 %～ 6 0 %范围内呈明显的线性关系 ,而肠道内液四环素的含量则明显降低 (P<0 .0 5 )。但肠道内容物量与冰糖浓度并无明显相关性。结论 :冰糖可明显增加大白鼠口服四环素在肠道的吸收 ;而冰糖浓度对肠腔液体量没有明显影响。结果提示冰糖增加口服四环素吸收可能通过主动吸收过程。

Primary Study on the Mechanism of Enhanced Absorption of Tetracycline by Crystal Sugar in the Rat Intestine

Aims:To study the mechanism by which crystal sugar enhances absorption of tetracycline in the rat intestine. Methods:Animal experiments were performed following the methods described by Fukahori at al;rats fasted for 24h were gavaged with tetracycline hydrochloride with or without concomitant of different concentrations of crystal sugar, i.e, 20%,40%,60% and 80% crystal sugar solution. Blood sample was collected then animal was sacrificed for intestinal content collection. Tetracycline in plasma and intestinal content was determined by fluorespectro photometry method. Results:Theoretical plasma peak concentrations of tetracycline were significantly elevated by coadministration of crystal sugar ( P <0.05),and the tetracycline remaining in the intestine decreased ( P <0.05),showing a well correlation with concentrations of crystal sugar used. The intestinal contents. however, did not show correlation with the concentrations of crystal sugar.Conclusions:Co administration of crystal sugar enhances absorption of tetracycline in rat intestine, and the intestinal content may not be affected by the concentrations of crystal sugar, indicating that the enhancing effect of crystal sugar on tetracycline absorption in intestine may be mediated by an active transport process.