脑脉通对栓塞性脑缺血大鼠动脉溶栓治疗时间窗的影响

目的观察脑脉通对血栓栓塞性脑缺血大鼠的保护作用及对溶栓治疗时间窗的影响。方法大鼠随机分为假手术组、模型组、尿激酶溶栓组(溶栓组)、脑脉通组、脑脉通联合尿激酶组(联合组)。自体血栓结合线栓阻塞大鼠大脑中动脉制备血栓栓塞性脑缺血动物模型。大鼠分别于缺血后3小时、6小时、9小时经导管进行区域动脉溶栓。动脉溶栓后24小时,观察大鼠脑组织病理损伤及神经症状,测定脑组织含水量和脑梗死面积变化。结果模型组大鼠均较假手术组神经症状评分增高,脑含水量增加,脑梗死面积增大,脑病理损伤明显;溶栓组大鼠神经症状评分降低、脑含水量减少、脑梗死面积减小、脑病理损伤减轻;6小时内溶栓的上述指标改善尤为明显;联合组各指标均较相应时间点溶栓组和脑脉通组的改善明显。结论不同时间窗经动脉溶栓治疗对大鼠脑缺血损伤均有保护作用,但缺血后3小时和6小时溶栓效果优于9小时;脑脉通可增强不同时间窗溶栓治疗的效果,具有延长溶栓治疗时间窗的作用。

Effect of Naomaitong on the Time Window of Thrombolysis through Artery for the Cerebral Ischemic Rats with Thrombo-Embolism

Objective To study the protective effect and influence of Naomaitong (Remove obstruction of cerebral vessels) on the time window of thrombolysis for the cerebral ischemic rats with thrombo-embolism. Methods The rats were randomized into sham-operated group, model group, urokinase (UK) thrombolysis group, Naomaitong group, and UK plus Naomaitong group (associated group). The cerebral ischemic rat models with thrombo-embolism were made by blocking sylvian artery. The rats were administrated local thrombolysis with duct to artery at the time-point of 3, 6, and 9h after cerebral ischemia. At 24h of administration through artery, the pathologic changes of brain and the symptoms of nerve system were observed to determine the brain water ratio (BWR) and the change of cerebral infarction area (CIA). Results Compared with the sham-operated group, in all model groups, the score of nerve system symptoms increased, the BWR and CIA increased, and the brain pathological changes became worse; in all thrombolysis groups, the score of nerve system symptoms decreased, the BWR and CIA decreased, and the brain pathologic changes became relieved; within 6 hours, the improvement of above-mentioned thrombolysis indices was especially remarkable; in the associated group, all indices at the corresponding time-point were improved significantly than those of the thrombolysis group and Naomaitong group. Conclusion Thrombolysis through artery given at different time is protective for the ischemic tissues, but the effect of treatment given at 3 and 6h after ischemia is better than that of 9h. Naomaitong enhances the protection, functioning to prolong the time window of thrombolysis.