紫杉醇对人宫颈癌细胞系HCE1影响的蛋白质组学

目的:研究紫杉醇作用于人宫颈癌细胞系HCE1细胞的分子机制.方法 : 以紫杉醇作用于人宫颈癌HCE1细胞后提取细胞总蛋白质,利用蛋白质组学技术对紫杉醇处理前后的HCE1细胞总蛋白质进行双向凝胶电泳图谱分析,通过质谱技术鉴定差异蛋白质.使用western 免疫印迹对其中2个蛋白质进行验证.结果 : 通过对紫杉醇处理前后宫颈癌细胞总蛋白质双向凝胶电泳图谱进行分析,找到差异蛋白质点42个.选择表达量差异较明显的21个蛋白质点进行质谱分析,15个蛋白质得到鉴定.这些蛋白质包括肽基脯氨酰顺反异构酶(PPIase A)、α-烯醇化酶(α-enolase)、角蛋白8(keratin 8)、热休克蛋白90(HSP90)以及真核细胞翻译起始因子(eIF-1A)等.结论 : 通过蛋白质组学技术,发现了紫杉醇处理前后宫颈癌细胞系HCE1差异蛋白质15个,这些差异蛋白质可能参与紫杉醇对宫颈癌细胞的抗癌作用.

Proteomic study of paclitaxel on human cervical carcinoma HCE1

OBJECTIVE: To explore the mechanism of paclitaxel on the protein expression of human cervical carcinoma cell line HCE1. METHODS: The total proteins extracted from paclitaxel-treated HCE1 cells were analyzed by 2-dimensional gel electrophoresis (2-DE), and compared with those from untreated HCE1 cells. The differential proteins were identified by mass spectrometry. Western blot was used to determine the differential expression levels of the 2 proteins. RESULTS: At 24 hour after paclitaxel (0.05 mumol/L) treatment, 2-DE images of paclitaxel-treated and paclitaxel-untreated cells were analyzed. Forty-two differential proteins were found. Twenty-one differential proteins among 42 proteins were analyzed by mass spectrometry, among which 15 proteins were identified, including peptidyl-prolylisomerases A (PPIase A),alpha-enolase,keratin 8,heat shock protein 90, eukaryotic translation initiation factor 1A, and so on. CONCLUSION: Fifteen proteins in human cervical carcinoma cells paclitaxel-treated and paclitaxel-untreated are found by proteomic techniques. These proteins may be involved in the proliferation inhibition of human cervical carcinoma cells by paclitaxel.