中药补阳还五汤对沙鼠脑缺血再灌注损伤及微循环障碍的治疗作用

目的 探讨中药补阳还五汤对脑缺血再灌注损伤的治疗作用及机制.方法 48只长爪沙鼠随机分成假手术组、脑缺血再灌注损伤模型组、补阳还五汤治疗组.夹闭沙鼠双侧颈总动脉45 min后松开夹子造成脑缺血再灌注损伤模型,干预组术前30 min、术后24 h后每天1次腹腔内注射补阳还五汤25.74 mg/kg,连续给药7 d,应用激光多普勒血流计观察脑组织血流量变化;单宁酸-氯化铁媒染标记微血管密度;伊文思蓝法标记微血管通透性;干湿法测定脑组织含水量;4-PTT旱路迷宫法(4-pellet taking test)评定沙鼠的学习记忆功能.结果 与模型组比较,补阳还五汤能够增加缺血1、5 min海马区血流量(P＜0.05),抑制再灌注15 min的急性高灌注,增加再灌注30、60、120 min延迟性低灌注期的血流量(P＜0.05);减轻脑缺血45 min再灌注48 h的脑微血管结构破坏程度,抑制血管通透性增加,减轻脑组织含水量(P＜0.05);4-PTT早路迷宫实验显示,补阳还五汤可改善沙鼠脑缺血再灌注损伤后的学习记忆能力(P＜0.05).结论 补阳还五汤对脑缺血再灌注损伤有很好的治疗作用,其机制与改善缺血再灌注损伤后的微循环障碍有关.

Effect of BuYangHuanWu Recipe on Cerebral Microcirculation in Gerbils with Ischemia-reperfusion

Objective To explore the protective mechanism of BuYangHuanWu recipe on neurofunction in gerbils with cerebral ischemia-reperfusion.Methods Gerbils(n=48)were divided randomly into three groups:animal model group,BuYangHuanWu recipe group,and sham control group.The animal model of cerebral ischemia was established using bilateral common carotid artery occlusion followed by unclamp 45 min after occlusion.The microcirculation was observed with a Laser Doppler.The density of microvascular was measured using Tannic acid ferric chloride mordant dyeing.The BBB(blood brain barrier)permeability was assessed using evan's blue(EB)dye.The water content in brain tissues was tested with wet and dry method.The learning and memory function test was performed with a 4-PTT dry path maze.Results Compared with the animal model group,BuYangHuanWu recipe increased blood flow in the hippocampal region at 1 and 5 min after occlusion,inhibited hypoperfusion at 15 min after reperfusion,increased blood flow at 30,60 and 120 min after reperfusion.Meanwhile,BuYangHuanWu recipe inhibited the increase of BBB permeability and water content in brain tissues after reperfusion(P＜0.05).BuYangHuanWu recipe also improved the scores of learning and memory function of the gerbils.Conclusion BuYangHuanWu recipe protects the neurofunction in gerbils with ischemia-reperfusionthrough modulating cerebral microcirculation damages.