仔猪金黄色葡萄球菌性心内膜炎研究

目的鉴定引起仔猪心内膜炎和内脏器官形成血栓的病原,并研究其引起内脏器官的组织学变化特征。方法应用生化鉴定、16S rDNA克隆测序和rDNA限制性酶切分析(ARDRA)等方法鉴定分离菌株,并进行致病性、药物敏感性和病理组织学试验。结果经鉴定病原为同源性较高的金黄色葡萄球菌(S.aureus);动物试验结果表明S201和S202毒力较强;药敏试验结果表明S201分离菌株对青霉素具有较强的耐药性;病理组织学检察发现内脏器官坏死、血栓和DIC变化。结论具有致病性的S.aureus能够引起仔猪心内膜炎等病理变化。

Endocarditis of piglets associated with Staphylococcus aureus

To identify the pathogen causing endocarditis and splanchyic thrombosis of piglets and to observe the histopathologic changes in the internal organs of the affected animals,the bacteria isolated was identified by cultural properties,biochemical reactions,16S rDNA sequencing and 16S rDNA restriction analysis（ARDRA）.In addition,pathogenicity and the drug-susceptibility of the pathogens isolated was determined by animal experiment and drug-sensitivity testing.It was found that the pathogen causing endocarditis in piglets was proved to be highly homologous Staphylococcus aureus which was hypersensitive to Aneet and Amikacin,but resistant to penicillin（S201）.As demonstrated by animal experiment,the strain S201 and S202 showed stronger pathogenicity to experimental animals in which necrosis in heart,liver,spleen and lymph node of lower jaw and thrombosis and DIC in heart,liver,spleen,lungs and lymph nodes could be demonstrated by histological examinations and the pathological changes were most prominent in heart.It is evident that highly homologous S.aurus can cause endocarditis,splanchnic necrosis,thrombosis and DIC in piglets.