中草药灯盏花对兔正畸牙移动过程中牙周组织血管内皮生长因子表达的影响

目的 检测中草药灯盏花对正畸牙牙周组织中血管内皮生长因子（VEGF）表达的影响，推测其加速正畸牙移动的可能作用机理。方法 45只兔分为A、B、C组，均以双侧下颌第一磨牙为实验牙。A组右侧第一磨牙离子导入灯盏花（A-R组），左侧导入生理盐水作为对照（A-L组）。B组安装使双侧下颌第一磨牙近中移动的正畸加力装置，同时右侧离子导入灯盏花（B-R组），左侧导入生理盐水作为对照（B-L组）。C组为空白对照组。A、B组分别于实验的1、3、7、14 d处死，每次处死5只兔。取所有动物的双侧下颌骨标本，B组测量牙移动距离后，所有标本制成第一磨牙的牙周组织切片，免疫组化法检测VEGF的表达。结果 B组第一磨牙移动距离在加力1、3、7、14 d时依次递增，B-R组在3、7、14 d的移动距离较B-L组大（P<0.01）。免疫组化染色表明C组和A-L组VEGF表达阴性，
A-R组、B-L组和B-R组表达较强，其中B-R组表达最强；A-R组和B-R组表达高峰在实验第3天，而B-L组表达高峰出现于第7天。结论 灯盏花可能通过上调正畸牙加力初期牙周组织中VEGF的表达而加速其移动。

Effect of Erigeron Breviscapus on the Expression of Vascular Endothelial Growth Factor in the Periodontal Tissues of Rabbits during Orthodontic Tooth Movement

Objective To detect the effect of erigeron breviscapus on the expression of vascular endothelial growth factor(VEGF) in the periodontal tissues during orthodontic tooth movement. Methods 45 rabbits were divided into 3 groups(groups A, B and C). Groups A and B included experimental group of 1, 3, 7 and 14 days respectively. The mandibular first molar of each experimental rabbit was observed. The rabbits of group A and group B received iontophoresis with erigeron breviscapus into the right(group A-R and group B-R) and with normal sodium into the left as the control(group A-L and group B-L). Additionally, the rabbits of group B were designed orthodontic appliance, by which 0.78 N mesial force was applied to pull the mandibular first molars. Group C, group of 0 day, was no iontophoresis and orthodontic appliance as the control. After killed on schedule, the amount of experimental tooth movement was measured and the expression of VEGF was examined by immunohistochemical method. Results The amount of experimental tooth movement increased successively from 1 to 14 days. The differences among days 3, 7 and 14 were significant in the comparison between group B-R and group B-L(P<0.01). The expression ofVEGF in groups A-R and B-L enhanced apparently compared with that in groups C and A-L(P<0.01), but that ingroup B-R was the most apparent(P<0.01). The expression of VEGF reached the peak level on day 3 in groups A-R and B-R(P<0.01), but it reached the peak level on day 7 in group B-L(P<0.01). Conclusion Erigeron breviscapus by iontophoresis can accelerate orthodontic tooth movement, and can meanwhile up-regulate the expression of VEGF in periodontium in the earlier period of orthodontic tooth movement. Thus it can be presumed that one of its mechanisms for erigeron breviscapus to accelerate orthodontic tooth movemen is erigeron breviscapus effects the metabolism and differentiation of osteoblast and osteoclast through up-regulating the expression of VEGF in periodontium.