Suppressive effects of Anoectochilus formosanus extract on osteoclast formation in vitro and bone resorption in vivo |
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Authors: | Kikuko Masuda Mayumi Ikeuchi Tomoyuki Koyama Kohji Yamaguchi Je-Tae Woo Tomio Nishimura Kazunaga Yazawa |
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Affiliation: | (1) Laboratory of Nutraceuticals and Functional Foods Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minatoku, Tokyo 108-8477, Japan;(2) Department of Biological Chemistry, Chubu University, Aichi, Japan;(3) Akatsuka Botanical Garden Co., Mie, Japan |
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Abstract: | Anoectochilus formosanus, a plant native to Taiwan, is used as a folk medicine. It was found that oral administration of A. formosanus extract (AFE) (500 mg/kg) for 4 weeks suppressed bone weight loss and trabecular bone loss in ovariectomized mice, an experimental model of osteoporosis. Although AFE at 12.5 and 25 μg/ml inhibited osteoclast formation in co-culture of osteoblasts and bone marrow cells, AFE did not inhibit the formation of osteoclast progenitor cells and preosteoclast cells in bone marrow cells and RAW264 cells. However, AFE (at 12.5 and 25 μg/ml) decreased RANKL expression. These results suggested that AFE might suppress the bone loss caused by estrogen deficiency through suppression of RANKL expression required for osteoclast formation. |
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Keywords: | Anoectochilus formosanus osteoporosis osteoclast bone resorption RANKL |
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