| P物质抗血清对小鼠病毒性心肌炎的作用研究 |
| |
| 引用本文： | 王勇,徐亚伟,李觉,陈欣,胡大一. P物质抗血清对小鼠病毒性心肌炎的作用研究[J]. 临床心血管病杂志, 2009, 25(7) |
| |
| 作者姓名： | 王勇 徐亚伟 李觉 陈欣 胡大一 |
| |
| 作者单位： | 同济大学附属第十人民医院心内科,上海,200072;上海同济大学心肺血管中心 |
| |
| 摘 要： | 目的:通过观察P物质抗血清对脑心肌炎病毒(EMCV)心肌炎的作用,探讨P物质在病毒性心肌炎发病机制中的作用.方法:4周龄DBA/2小鼠经腹腔接种EMCV制成病毒性心肌炎模型,腹腔注射P物质抗血清观察其对小鼠病毒性心肌炎的作用.观察小鼠在接种EMCV后14 d生存率的变化.小鼠心肌切片苏木精-伊红染色、Masson trichrome染色,观察心室肌坏死和炎症细胞浸润面积的变化.甲苯胺蓝(TB)染色显示心室肌组织内肥大细胞,观察肥大细胞密度的变化.荧光定量RT-PCR检测小鼠心脏促炎细胞因子[白细胞介素-6(IL-6),肿瘤坏死因子-α(TNF-α)]、基质金属蛋白酶(MMP-9)和肥大细胞蛋白酶类胰蛋白酶及糜蛋白酶(mMCP-4, mMCP-5, mMCP-6) mRNA表达.ELISA法检测小鼠心脏IL-6、TNF-α及MMP-9的表达.结果:P物质抗血清治疗使小鼠的14 d生存率明显改善(80%: 30%, P<0.05);小鼠心脏/体重比值显著改善(P<0.01);小鼠左心室坏死和炎症细胞浸润面积明显减少(P<0.01);左心室肥大细胞密度明显降低(P<0.01);IL-6、TNF-α、MMP-9、mMCP-4、mMCP-5及mMCP-6 mRNA的表达明显下调(P<0.05);小鼠心脏IL-6, TNF-α及MMP-9蛋白质表达减少(P<0.05).结论:P物质抗血清可改善EMCV小鼠病毒性心肌炎的心肌炎症和生存率,下调心肌促炎细胞因子和基质金属蛋白酶的表达,降低肥大细胞密度及其特异蛋白酶类胰蛋白酶和糜蛋白酶的表达,表明P物质可能在病毒性心肌炎急性炎症和心室重构中起重要作用.
|
| 关 键 词： | 心肌炎 P物质 肥大细胞 |
The effect of anti-substance P serum on viral myocarditis in mice |
| |
| WANG Yong,XU Yawei,LI Jue,CHEN Xin,HU Dayi. The effect of anti-substance P serum on viral myocarditis in mice[J]. Journal of Clinical Cardiology, 2009, 25(7) |
| |
| Authors: | WANG Yong XU Yawei LI Jue CHEN Xin HU Dayi |
| |
| Affiliation: | 1Department of Cardiology;No.10 People's Hospital;Tongji University;Shanghai;200072;China;2Heart;Lung and Blood Vessel Center;Tongji University |
| |
| Abstract: | Objective:This study the role of substance P in murine viral myocarditis induced by encephalomyocarditis virus(EMCV)by the observing the effect of anti-substance P serum on it.Method:Four-week old DBA/2 mice were inoculated intraperitoneally with EMCV.EMCV infected mice were injected intraperitoneally with anti-substance P serum.The 14-day survival of mice was determined.Pathological study was performed to evaluate necrosis areas and inflammatory infiltration in murine hearts.TB staining was performed to ev... |
| |
| Keywords: | myocarditis substance P mast cells |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
