原发性肝细胞癌中DNMT3b基因剪接变异体的表达分析

目的:研究原发性肝癌中DNMT3b基因剪接变异体的表达情况及其在肝癌发生发展中的作用。方法:通过RT-PCR的方法对30例肝癌、30例癌旁组织中DNMT3b基因的剪接变异体进行检测,并将结果与肝癌患者临床指标进行统计学分析。结果:RT-PCR结果显示DNMT3b基因以四种剪接变异体(DNMT3b1、DNMT3b3、DNMT3b4和DNMT3b5)的形式表达,其总的表达率肝癌组为93.3%(28/30),癌旁组为93.3%(28/30)。其中DNMT3b4在肝癌组中的表达高于癌旁组(P<0.05),与肝癌病理分化和CLIP评分无相关性(P>0.05)。结论:DNMT3b基因剪接变异体DNMT3b4可能参与肝癌的发生演变。

Analysis of Splice Variants of DNMT3b Gene in Primary Hepatocellular Carcinoma

Objective: To study the splice variants of DNMT3b gene in primary hepatocellular carcinoma,and their effects on the development of hepatocellular carcinoma. Methods: Thirty hepatocellular carcinoma(HCC) tissues and their corresponding adjacent para-cancerous tissues were examined. RT-PCR was used to detect the splice variants of DNMT3b gene. The relationship between the laboratory results and clinicopathological characteristics of HCC was analyzed as well. Results: The expressions of DNMT3b gene were detected by RT-PCR in the forms of alternative spliced variants of DNMT3b1,DNMT3b3,DNMT3b4 and DNMT3b5. Their total expressions were 93.3%(28/30) in HCC and 93.3%(28/30)in para-cancerous tissues. There was a higher DNMT3b4 expression in HCC than that in para-cancerous tissues(P<0.05). There was no relationship between pathological differentiation and CLIP grade(P>0.05). Conclusion: DNMT3b4,one of the alternative spliced forms of DNMT3b,may play an important role in hepatic carcinogenesis.