| Effect of Mouse Oocyte Vitrification on Mitochondrial Membrane Potential and Distribution简 |
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| 引用本文： | 雷涛,郭娜,谭美华,李豫峰. Effect of Mouse Oocyte Vitrification on Mitochondrial Membrane Potential and Distribution简[J]. 华中科技大学学报(医学英德文版), 2014, 34(1): 99-102. DOI: 10.1007/s11596-014-1238-8 |
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| 作者姓名： | 雷涛 郭娜 谭美华 李豫峰 |
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| 基金项目： | supported by the Foundation of Hubei Provincial Health Department,China（No.JX5A02） |
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| 摘 要： | The effects of mouse oocyte vitrification on mitochondrial membrane potential and distribution were explored in this study. The collected mouse oocytes were randomly divided into vitrification and control groups. Ethylene glycol（EG） and dimethylsulphoxide（DMSO） were used as cryoprotectants in the vitrification group. The mitochondrial function and distribution in the oocytes were examined by using the fluorescent probes, JC-1 and Mito Tracker green. The results showed that the ratio of red to green fluorescence in mouse oocytes was significantly decreased after thawing in the vitrification group as compared with the control group（1.28 vs. 1.70, P0.05）. The percentage of polarized distribution of the mitochondria in oocytes was conspicuously reduced in the vitrification group when compared with the control group（31% vs. 63%, P0.05）. It was suggested that vitrification significantly affects the mitochondrial function and distribution in oocytes and reduces the potential of oocyte fertilization and embryo development.
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| 关 键 词： | 小鼠卵母细胞 线粒体膜电位 玻璃化冷冻 冷冻保护剂 二甲基亚砜 和分布 对照组 DMSO |
Effect of mouse oocyte vitrification on mitochondrial membrane potential and distribution |
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| Tao Lei,Na Guo,Mei-hua Tan,Yu-feng Li. Effect of mouse oocyte vitrification on mitochondrial membrane potential and distribution[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2014, 34(1): 99-102. DOI: 10.1007/s11596-014-1238-8 |
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| Authors: | Tao Lei Na Guo Mei-hua Tan Yu-feng Li |
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| Affiliation: | 1. Department of Reproductive Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China
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| Abstract: | oocytes vitrification mitochondria mice |
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| Keywords: | oocytes vitrification mitochondria mice |
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