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| Dioscin-induced Apoptosis of Human LNCaP Prostate Carcinoma Cells through Activation of Caspase-3 and Modulation of Bcl-2 Protein Family简 | |
| 引用本文： | 陈静,李辉敏,张学农,熊朝梅,阮金兰.Dioscin-induced Apoptosis of Human LNCaP Prostate Carcinoma Cells through Activation of Caspase-3 and Modulation of Bcl-2 Protein Family简[J].华中科技大学学报(医学英德文版),2014,34(1):125-130. |
| 作者姓名： | 陈静 李辉敏 张学农 熊朝梅 阮金兰 |
| 作者单位： | [1]Key Laboratory of Natural Medicinal Chemistry and Resources Evaluation of Hubei Province, School of Pharmacy, TongjiMedical College, Huazhong University of Science and Technology, Wuhan 430030, China [2]School of Basic Medical Science, Jiujiang University, Jiujiang 332000, China [3]Department of Pharmacy, Hchang Central People ＂s Hospital ＆ the First College of Clinical Medical Science, China Three Gorges University, Yichang 443003, China |
| 基金项目： | supported by the National Natural Science Foundation of China（No.81173065） |
| 摘 要： | Dioscin is a natural steroid saponin derived from several plants, showing potent anti-cancer effect against a variety of tumor cell lines. In the present study, we investigated the anti-cancer activity of dioscin against human LNCaP cells, and evaluated the possible mechanism involved in its antineoplastic action. It was found that dioscin（1, 2 and 4 μmol/L） could significantly inhibit the viability of LNCaP cells in a time- and concentration-dependent manner. Flow cytometry revealed that the apoptosis rate was increased after treatment of LNCaP cells with dioscin for 24 h, indicating that apoptosis was an important mechanism by which dioscin inhibited cancer. Western blotting was employed to detect the expression of caspase-3, Bcl-2 and Bax in LNCaP cells. The expression of cleaved caspase-3 was significantly increased, and meanwhile procaspase-3 was markedly decreased. The expression of anti-apoptotic protein Bcl-2 was down-regulated, whereas the pro-apoptotic protein Bax was up-regulated. Moreover, the Bcl-2/Bax ratio was drastically decreased. These results suggested that dioscin possessed potential anti-tumor activity in human LNCaP cells through the apoptosis pathway, which might be associated with caspase-3 and Bcl-2 protein family. |
| 关 键 词： | Bcl-2蛋白 Caspase-3 薯蓣皂甙 细胞凋亡 前列腺癌细胞 Bcl-2 Bax caspase-3 LNCaP细胞 |
Dioscin-induced apoptosis of human LNCaP prostate carcinoma cells through activation of caspase-3 and modulation of Bcl-2 protein family |
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| Jing Chen,Hui-min Li,Xue-nong Zhang,Chao-mei Xiong,Jin-lan Ruan.Dioscin-induced apoptosis of human LNCaP prostate carcinoma cells through activation of caspase-3 and modulation of Bcl-2 protein family[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2014,34(1):125-130. | |
| Authors: | Jing Chen Hui-min Li Xue-nong Zhang Chao-mei Xiong Jin-lan Ruan |
| Institution: | 1. Key Laboratory of Natural Medicinal Chemistry and Resources Evaluation of Hubei Province, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China 2. School of Basic Medical Science, Jiujiang University, Jiujiang, 332000, China 3. Department of Pharmacy, Yichang Central People’s Hospital & the First College of Clinical Medical Science, China Three Gorges University, Yichang, 443003, China |
| Abstract: | dioscin LNCaP anti-tumor apoptosis pathway capsase-3 Bcl-2 protein family |
| Keywords: | dioscin LNCaP anti-tumor apoptosis pathway capsase-3 Bcl-2 protein family |
| 本文献已被 CNKI 维普 SpringerLink 等数据库收录！ | |