| 风疹病毒的分离 |
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| 引用本文： | 韩世杰,王桂亭,周树安,姚苹. 风疹病毒的分离[J]. 山东大学学报(医学版), 1985, 0(2) |
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| 作者姓名： | 韩世杰 王桂亭 周树安 姚苹 |
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| 作者单位： | 山东医学院病毒研究室，山东医学院病毒研究室，山东医学院病毒研究室，山东医学院病毒研究室 |
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| 摘 要： | 自24例风疹症状典型的患儿咽拭中,经PRK细胞盲传后转种至RK_(13)及BhK_(21)细胞上出现明显的细胞病变,在MK_2细胞上对HSV-1型病毒攻击呈干扰现象者为阳性,分离出6株风疹病毒,命名为济南风疹病毒JRV_5、JRV_(11)、JRV_(13)、JRV_(18)JRV_(20)及JRV_(23)。
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| 关 键 词： | 风疹病毒 酶连接免疫吸附测定 |
ISOLATION OF RUBELLA VIRUS |
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| Han Shijie et al. ISOLATION OF RUBELLA VIRUS[J]. Journal of Shandong University:Health Sciences, 1985, 0(2) |
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| Authors: | Han Shijie et al |
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| Abstract: | Six strains of rubella virus were isolated from the pharyngeal swabs of 24 clinically diagnosed rubella patients. These viruses cause marked cytopathic effect in RK-13 and BHK-21 cell lines and the LLC-MK 2 cells infected with them show interference with the invasion of HSV-1. Immunofluorscence and enzyme-linked immunosorbent assay were used to identify the viruses, to trace and to localize their antigens in cell culture. Neutralization, hemagglutination and hemagglutination inhibition tests were applied to further confirm these newly isolated rubella viruses. |
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| Keywords: | Rubella virus Enzyme-linked immunosorbent assay |
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