| Construction of Expressing Plasmids of Recombinant FN Polypeptides with Bifunctional－domain and the Characterization of the P |
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| 引用本文： | 冯作化,张桂梅,李东,张慧.Construction of Expressing Plasmids of Recombinant FN Polypeptides with Bifunctional－domain and the Characterization of the P[J].华中科技大学学报(医学英德文版),1996(2). |
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| 作者姓名： | 冯作化 张桂梅 李东 张慧 |
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| 作者单位： | Department of Medical Molecular Biology，Tongji Medical University，Wuhan 430030 |
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| 摘 要： | ＣｏｎｓｔｒｕｃｔｉｏｎｏｆＥｘｐｒｅｓｓｉｎｇＰｌａｓｍｉｄｓｏｆＲｅｃｏｍｂｉｎａｎｔＦＮＰｏｌｙｐｅｐｔｉｄｅｓｗｉｔｈＢｉｆｕｎｃｔｉｏｎａｌ－ｄｏｍａｉｎａｎｄｔｈｅＣｈａｒａｃｔｅｒｉｚａｔｉｏｎｏｆｔｈｅＰｒｏｄｕｃｔｓＥｘｐｒｅｓｓｅ...
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Construction of Expressing Plasmids of Recombinant FN Polypeptides with Bifunctional-domain and the Characterization of the Products Expressed in E. Coli |
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| FENG Zuohua,ZHANG Guimei,LI Dong,ZHANG Hui.Construction of Expressing Plasmids of Recombinant FN Polypeptides with Bifunctional-domain and the Characterization of the Products Expressed in E. Coli[J].Journal of Zuazhong University of Science and Technology: Medical Edition,1996(2). |
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| Authors: | FENG Zuohua ZHANG Guimei LI Dong ZHANG Hui |
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| Abstract: | Two expressing plasmids have been constructed and used to express two bifunctional-domain recombinant polypeptides of human fibronectin (FN) in E. coli. One was CH50 (Pro1239-Ser1515 of FN linked with Ala1690-Thr1960 of FN through Met) and the other was CH56 (Pro1239-Thr1960 of FN). Both of two polypeptides were capable of binding heparin and were purified by heparin-a-garose affinity chromatography. The purified products were capable of binding cells. The production of CH50 and CH56 polypeptides provided a fundamental basis for further study of the anti-metastatic function of recombinant fibronectin polypeptides. |
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| Keywords: | fibronectin recombinant polypeptide metastasis |
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