不同时辰和频率电针对大鼠隔外侧核生长抑素mRNA阳性神经元的影响

目的 :观察不同时辰低频 ( 2Hz)和高频 ( 1 2 8Hz)电针对大鼠隔外侧核 (LSN)生长抑素(SOM)mRNA阳性神经元表达的影响。方法 :5时、1 1时、1 7时及 2 3时分别用 2Hz和 1 2 8Hz电针大鼠一侧“环跳”穴后 ,采用原位杂交组织化学方法显示LSN内SOMmRNA阳性神经元表达 ,并与对照组比较。结果 :针刺大鼠一侧“环跳”穴后 ,1 1时和 1 7时 2Hz电针组及 5时、1 7时和 2 3时 1 2 8Hz电针组 (LSN)内SOMmRNA阳性神经元的数量均比对照组增多 (P <0 .0 5或P <0 .0 1 ) ;5时、1 7时和 2 3时 1 2 8Hz电针组 (LSN)内SOMmRNA阳性神经元的数量比 2Hz电针组增多 (P <0 .0 5或P <0 .0 1 ) ;1 7时 1 2 8Hz电针组 (LSN)内SOMmRNA阳性神经元的数量比 5时、1 1时和 2 3时均明显增多 (P <0 .0 5或P <0 .0 1 )。结论 :电针“环跳”穴对大鼠 (LSN)内SOMmRNA阳性神经元的表达与时间和频率有关。

Effect of Electroacupuncture at Different Time and Different Frequencies on Somatostatin mRNA Expression Positive Neurons of the Lateral Septal Nucleus in the Rat

Objective:To observe the effect of electroacupunc ture (EA) at different tim e and frequencies on somatostatin (SOM)mRNA expression positive neurons in the l ateral septal nucleus (LSN) in rats. Methods: A total of 60 SD rats were randomi zed into control, 2 Hz-EA and 128 Hz-EA groups which were further divided int o 5-o ’clock, 11-o’clock, 17-o’clock and 23-o’clock subgroups. Unilateral “Hu anti ao”(GB 30) was punctured and stimulated electrically by setting the parameters being strength of 3 V, wave-width of 1 ms, frequencies of 2 Hz and 128 Hz, and stimu lating duration of 30 min. After acupuncture treatment, the rats were killed for taking out the brain samples. Following freezing, the brain tissue was cut into sections (40 μm) and the in situ hybridization histochemical method was used for displaying SOM mRNA expression. The number of SOM mRNA expression positive neuro ns of LSN (4 sections/rat) was calculated under light microscope. Re sults: After EA of “Huantiao”(GB 30), compared with control group, SOM mRNA positive neurons in LSN of 2 Hz-EA group at 11- and 17-o’clock and 128 Hz-EA group at 5-, 1 7- and 23-o’clock increased significantly in the number (P<0.05～0.01). Com parison between 2 Hz- and 128 Hz-EA groups showed that SOM mRNA positive neur ons in LSN of the later group at 5-,17- and 23-o’clock were markedly more tha n those of the former group at the same time courses, suggesting that the effect of 128 Hz EA on SOM mRNA expression was apparently stronger than that of 2 Hz E A. In 128 Hz-EA group, comparison among different time courses, SOM mRNA positive neu rons at 17-o’clock were markedly more than those of 5-,11- and 23-o ’clock, indicating that the effect of EA at 17-o’clock on SOM mRNA expressi o n was stronger than those of EA in the other time courses.Conclusion: EA stimulation in duced increase of the number of SOM mRNA expression positive neurons in the LSN h as a close association with the time and frequencies of EA in rats.