犬心脏表面主要神经节丛VIP免疫组化研究

目的：探讨犬心脏表面神经节丛的化学性质。方法：用恒冷箱切片和免疫组化ABC法对犬心脏表面主要神经节丛进行了血管活性肠肽（VIP）免疫组化研究。结果：在犬心房和心室表面各神经节丛内均见VIP-IR神经元，分布广、数量多。心房表面神经节丝内的VIP-IR神经元都较心室表面神经节丛内显著多。在心脏表面各脂肪垫发现大量VIP-IR神经纤维，多靠近血管走行或附于血管壁，个别部位见这种肽能神经纤维似与心肌细胞接触。结论：犬心脏表面神经节丛内确实存在VIP，这种肽能神经对心房和心室的支配不对称，VIP能神经元在犬心脏内可能执行与其他肽能神经元不同的功能。从形态学角度提示，VIP可能直接参与心肌细胞和心脏血管活动的调控。

Immunohistochemical Research on VIP in the Canine Main Superficial Cardiac Ganglia Plexus

Objective: In order to investigate the chemical properties of superficial cardiac neurons. Methods: By means of immunohistochemical ABC technique, the study was performed concerning the distribution of Vasoactive Intestinal Peptide(VIP) in the canine main cardiac superficial ganglia plexus. Results: VIP-IR neurons were found in every ganglia plexus observed. In contrast with CGRP-IR and SP-IR neurons, the shape and size of VIP-IR neurons were smaller, but the number is larger. The comparative study on atria and ventricles indicated that ther were more VIP-IR neurons in atria than in ventricles. Numerous VIP-IR nerve fibers could observed in the each fat pats and intermyocardiocytes. These nerve fibers usually situated near blood vessels, or were attached to vessel wall. Somewhat VIP-IR nerve fibers connected with myocardiocytes in some regions. Conclusion:There actualy exists VIP in canine cardiac superficial ganglia plexus. The VIP-IR neurons were likely to perform different functions in canine heart. The present study implied that the regulation of the three kinds of peptide neurons to atria and ventricle was different, and in morphology VIP could modify the activities of myocardiocytes and vessels of heart directly.