幼年雄性SD大鼠营养性肥胖模型的建立

目的建立一种贴近临床的幼年雄性SD大鼠营养性肥胖模型。方法用高能量饲料喂养幼年大鼠4～8周,观察大鼠体重及肥胖指数、内脏脂肪及脂肪系数、血脂和血浆瘦素水平的变化。结果造模第4周末,高能量饲料组大鼠体重已明显大于基础饲料对照组,肥胖指数、内脏脂肪质量及脂肪系数也大于对照组,同时血浆甘油三酯、总胆固醇及低密度脂蛋白胆固醇均明显增高,高密度脂蛋白胆固醇呈明显降低趋势,空腹血浆瘦素明显升高;造模第8周末,模型组体重增加更明显,上述其他各项指标异常进一步加重。结论高能量饮食可以使幼年大鼠发生营养性肥胖,并伴有高脂血症。

Establishment of Diet-Induced Obesity Model of Impuberism Male SD Rat

Objective To establish the impuberism male SD rat model with nutritional obesity.Methods Impuberism male SD rats were divided into two groups and maintained for 4 weeks to 8 weeks on high-energy diet and normal diet respectively.Both groups＇ body weight,Lee＇s index,abdominal fat,plasma TG,TC,LDL-C and HDL-C,plasma leptin levels were measured after 4-8 weeks.Results Compared with the normal feeding group,the body weight in the high-energy feeding group significantly increased,Lee＇s index and abdominal fat were also more than those in the control group,the levels of plasma TG,TC,LDL-C and leptin remarkably increased,while the plasma HDL-C lowered.The above abnormal changes became even worse at the end of 8 weeks.Conclusion Impuberism rats fed high energy diet can develop nutritional obesity with high plasma TG,TC,LDL-C and leptin and lower HDL-C level.